VEGF、sflt-1在子宫内膜异位症患者血清中表达的临床研究

Expression of VEGF and sflt-1 in Serum of Patients with Endometriosis

目的:探讨血管内皮生长因子(VEGF)、可溶性血管内皮生长因子受体1(sflt-1)在子宫内膜异位症(EMT)发病中的作用及sflt-1在EMT诊断中的价值。方法:随机选择本院经腹腔镜或开腹手术后病理检查证实为EMT的患者40例(EMT组)和体检无异常的健康妇女20例为对照组。采用酶联免疫吸附法测定两组血清中VEGF及sflt-1的浓度,并计算VEGF活性指数及sflt-1的ROC曲线下面积,分析VEGF、sflt-1、VEGF活性指数与EMTr-AFS分期及痛经程度的相关性。结果:①EMT组血清中VEGF、sflt-1的浓度及VEGF活性指数均明显高于对照组(P<0.01);②EMT组血清中VEGF浓度及VEGF活性指数与r-AFS分期及痛经程度呈正相关(P<0.01),而sflt-1浓度与r-AFS分期及痛经程度呈负相关(P<0.01);③EMT组血清中sflt-1的ROC曲线下面积为0.912。结论:VEGF、sflt-1与EMT的发病相关;采用VEGF活性指数可更全面了解VEGF在EMT中的作用;sflt-1对EMT的血清学诊断有一定的价值。

Objective：To study the action of vascular endothelial growth factor （VEGF） and soluble vascular endothelial growth factor receptor 1 （sflt-1） in the pathogenesis of endometriosis （EMT）,and the value of sflt-1 in the diagnosis of endometriosis.Methods：40 patients with EMT diagnosed by pathology after laparoscopy or laparotomy were randomly chosen as EMT group and 20 healthy women were as control group.The levels of VEGF and sflt-1 in serum were detected by ELISA in two groups.VEGF activity indices and ROC Area Under Curve of sflt-1 were calculated.The correlation between VEGF,sflt-1,VEGF activity indices and the classification （r-AFS）,the severity of dysmenorrhea were analyzed respectively.Results：① In EMT group,the concentrations of VEGF and sflt-1 and VEGF activity indices in serum were elevated significantly compared with those in the control group（P0.01）;②In EMT group,the concentrations of VEGF and VEGF activity indices in the serum were positively correlated with r-AFS and dysmenorrhea（P0.01）,but a negative correlation was found between the concentrations of sflt-1 and r-AFS and dysmenorrhea（P0.01）,③ The ROC Area Under Curve of sflt-1 was 0.912.Conclusions： VEGF and sflt-1 are closely related to the pathogenesis and progression of EMT.We can have a thorough knowledge of the action of VEGF on EMT by VEGF activity index.Sflt-1 is valuable to the serological diagnosis of EMT.