阿霉素预处理的小鼠红白血病细胞降低红白血病细胞的成瘤性

Decreasing tumorigensis of mouse erthroleukemic cells by pre-infusion of adriamycin-treated erthroleukemic cells in mice

目的:研究阿霉素(ADM)处理的小鼠红白血病(MEL)细胞是否可以发生免疫原性死亡,预先输注此细胞是否可以降低MEL细胞在昆明(KM)小鼠体内的成瘤性。方法:用ADM诱导MEL细胞发生约70%凋亡率,即发生免疫原性死亡。30只KM小鼠分3组,分别为小鼠红白血病模型组(模型组)、ADM诱导治疗组(ADM组)和空白对照组(对照组)。模型组经尾静脉输注PBS给KM小鼠,8 d后输注MEL细胞1×106/只;ADM组经尾静脉输注ADM处理的MEL细胞给KM小鼠,9×106/只,8 d后输注MEL细胞1×106/只;对照组未行任何人为干预。观察各组小鼠的生存时间、濒死或第80 d时外周血白细胞数目和体重。结果:在体外使用4 mg/L阿霉素作用于MEL细胞24 h后,诱导MEL细胞约70%凋亡率,发生了免疫原性死亡。在小鼠体内,观察80 d,对照组小鼠全部存活,模型组全部成瘤死亡,ADM组有1只长期生存。ADM组与模型组相比较,生存时间延长(P<0.01),濒死或第80 d时外周血白细胞数目降低(P<0.01),濒死或第80 d时体重增加(P<0.01)。结论:将发生免疫原性死亡的MEL细胞预先输注到KM小鼠体内,KM小鼠产生抗肿瘤免疫应答,降低了活性MEL细胞在KM小鼠体内的成瘤性,改善KM小鼠生存时间和生存质量。

AIM：To investigate the effect of decreasing tumorigensis of mouse erthroleukemic（MEL） cells by pre-infusion of adriamycin（ADM）-treated MEL cells in mice.METHODS： The immungentic cell death of MEL cells was prepared by pre-treatment with ADM in vivo.The Kunming（KM） mice were divided into 3 group： MEL model group,ADM treatment group and control group.The mice in MEL model group were intravenously injected with PBS on the first day,and then with 1×106 MEL cells on the 8th day.The mice in ADM treatment group were intravenously injected with 9×106 MEL cells pretreated with ADM on the first day,and then with 1×106 MEL cells without ADM pretreatment on the 8th day.The mice in control group didn＇t receive any intervention on the first day and 8th day.The survival time,the white cell count in peripheral blood and body weight were observed.RESULTS： All mice in control group were survived.All mice in MEL model group died from erythroleukemia.Only 1 mouse in ADM treatment group survived on the 80th day.The survival time in control group,MEL model group and ADM treatment group was 80 d,（28.00±8.43） d and（56.00±12.68） d,respectively.The survival time in ADM treatment group was longer than that in MEL model group（P0.01）.The peripheral blood white cell count in ADM treatment group was lower than that in ADM model group（P0.01）.The body weight of the mice in ADM treatment group on the 80th day was higher than that in MEL model group（P0.01）.CONCLUSION： The pre-infusion of adriamycin-treated MEL cells in erythroleukemic mice attenuates the tumorigenesis of MEL cells and improves survival time and life quality.