人参皂苷Rh_2对肝癌SMMC-7721细胞增殖和细胞骨架的影响

Effect of ginsenoside Rh_2 on cell proliferation and cytoskeleton in hepatocarcinoma SMMC-7721 cells

目的:探讨人参皂苷Rh2(Rh2)对人肝癌细胞SMMC-7721细胞生长和细胞骨架的影响。方法:应用MTT法及流式细胞仪检测不同浓度Rh2对SMMC-7721细胞增殖的抑制及诱导凋亡作用;激光共聚焦显微镜观察细胞骨架肌动蛋白F-actin的分布状态;整装细胞电镜术观察细胞核基质-中间纤维系统的变化。结果:MTT法和流式细胞仪分析结果分别显示,40 mg/L Rh2作用SMMC-7721细胞4 d时的抑制率及细胞凋亡率明显高于对照组及10、20 mg/L组,显著差异(P<0.05);激光共聚焦显微镜下,SMMC-7721细胞经Rh2处理4 d,F-actin分布均匀,细丝排列整齐,数量增多;而对照组细胞F-actin细丝在细胞内排列紊乱,多呈斑点状分布。整装电镜结果显示,与对照组相比,实验组细胞的中间纤维丰富,均匀地满布细胞中,呈网架状连接的结构。结论:Rh2能有效抑制SMMC-7721细胞的增殖,增加SMMC-7721细胞的凋亡,并且诱导SMMC-7721细胞骨架发生变化。

AIM：To investigate the changes of cell growth and cytoskeleton in hepatocarcinoma SMMC-7721 cells treated with ginsenoside Rh2.METHODS： Cell viability and apoptosis under the conditions of ginsenoside Rh2 exposure at different concentrations were measured by MTT test and flow cytometry,respectively.The morphological changes of F-actin labeled with FITC-phalloidin were observed under confocal laser scanning microscope.The structures of nuclear matrix-intermediate fibre system were observed under transmission electron microscope（TEM）.RESULTS： Rh2 at 40 mg/L for 4 days inhibited the proliferation and induced apoptosis in SMMC-7721 cells more than those in control group,10 mg/L Rh2 group and 20 mg/L Rh2 group.The F-actin in the cells treated with Rh2 was well-distributed,lined up in order and the number of fibers increased,while those in the control cells were in disorder and punctiform.The results of whole mount TEM indicated that the intermediate fiber was plentiful,well-distributed and interweaved into a regular network in Rh2 treated cells.CONCLUSION： Rh2 effectively inhibits the cell proliferation,increases the cell apoptosis and induces the change of the cytoskeleton alignment in SMMC-7721 cells.