载脂蛋白B、载脂蛋白E及血管紧张素原基因多态性与冠心病的关系

The Research of Apolipoprtein B,E and Angiotensinogen Gene Polymorophisms in Predisposition to CHD

目的探讨载脂蛋白B、E(ApoB、ApoE)基因及血管紧张素原基因(AGT)多态性与冠心病(CHD)的相关性。方法采用基因芯片技术分析89例CHD患者和78例非CHD患者的ApoB Xba I、ApoE112/158及AGT M235T基因多态性及等位基因频率。结果 CHD组ApoB Xba I、ApoE112/158及AGT M235T基因型分布与对照组相比差异有统计学意义(P<0.05)。CHD组的ApoB Xba I的X+X+基因型及等位基因X+频率显著高于对照组,差异有统计学意义(P<0.05),ApoE112/158的ε3/4、ε4/4基因型及等位基因ε4频率高于对照组,差异有统计学意义(P<0.05);CHD组AGT-TT基因型频率及T等位基因频率均明显高于对照组,差异有统计学意义(P<0.05)。结论 ApoB Xba I、ApoE112/158和AGT M235T的基因多态性可能是中国人CHD的危险因素。ApoB Xba I等位基因X+;ApoE112/158的等位基因ε4和AGT M235T基因的T等位基因是CHD的重要遗传标记。

Objective To explore the relationship of apolipoprotein B, E （ ApoB, ApoE ） and angiotensinogen （ AGT ） gene polymorphism with coronary heart disease （ CHD ）. Methods Gene chip technology was performed to analyze ApoB Xba I , ApoE 112/158 and angiotensinogen gene polymorphism. The allele frequency and gene type distribution were compared between groups. Results There was a significant difference in the distributuion of Apo B,.ApoE and AGT gene type between patients and controls （ P 〈 0.05 ）. The frequency of X^＋X^＋ genetype and X＋ allele of ApoB were significantly higher in patients with CHD than controls（ P 〈 0.05 ） ; the frequency of ε 3/4, ε 4/4 genetype and e 4 allele of ApoE112/158 were higher in patients with CHD than controls （P 〈 0.05 ）. AGT-TT gene type was related with CHD significantly （ P = 0.003 ） and T allele was more frequent significantly in CHD group than controlls （P 〈 0.05 ）. Conclusion The Apo B, ApoE and AGT gene polymorphism may be the risk factors of CHD; X^＋ allele of ApoB Xba I and e 4 allele of ApoE gene and T allele of AGT gene might be the geneticmarkcrs of CHD.