磷酸化Akt-1在胃癌中的表达及其临床意义

Expression of pAkt-1 and clinical significance in gastric carcinoma

目的研究Akt-1在胃癌中的磷酸化情况,探讨其与胃癌发生发展、治疗及预后的关系。方法利用免疫组化法检测108例胃癌组织中pAkt-1的表达情况,并结合临床资料进行分析。结果胃癌组织中pAkt-1的高表达率为50.9%,pAkt-1的高表达与肿瘤大小、病理类型及临床分期相关。在生存分析中,pAkt-1高表达及临床分期是胃癌患者术后无疾病生存期(DFS)的独立预后因素。对pAkt-1高表达患者,未使用多西紫杉醇者术后中位DFS为39.0个月,显著长于使用多西紫杉醇者的11.1个月(P=0.005);对pAkt-1低表达者,是否使用多西紫杉醇对DFS无显著影响(P=0.745)。结论 pAkt-1在胃癌中存在高表达,可能参与胃癌的发生、发展与复发。pAkt-1高表达可能与患者对多西紫杉醇耐药相关。

Objective To study the expression of pAkt-1 on gastric carcinoma and to discuss its relationship with the development process and postoperative prognosis in gastric carcinoma.Methods Immunohistochemical method was used to detect the expression of pAkt-1 in 108 specimens of gastric carcinoma,and clinical data were also studied.Results The high expression rate of pAkt-1 was 50.9% in gastric carcinoma tissue.Its high expression was related with tumor size,differentiation and clinical stage.In survival analysis,the expression of pAkt-1 and clinical stage were significantly related with disease free survival（DFS）.In pAkt-1 high expression patients,the DFS of those treated with docetaxel（11.1months） was much shorter than patients treated without docetaxel（39.0 months）.However,the DFS did not vary too much in the group of pAkt-1 low expression no matter whether docetaxel was applied.Conclusion The over-expression of pAkt-1 may play a role in the pathogenesis,metastasis and recurrence of gastric carcinoma.The over-expression of pAkt-1 may be associated with resistance of docetaxel.