虫草菌液对高糖作用下大鼠腹膜间皮细胞转化生长因子β_1和纤维连接蛋白表达的影响

Effects of Hirsutella Sinensis on the Expression of Transforming Growth Factor-β_1 and Fibronectin in Rat Peritoneal Mesothelial Cells Cultured in High Glucose Concentration

目的:研究虫草菌液(HS)对高糖作用下体外培养的大鼠腹膜间皮细胞(RPMCs)转化生长因子β1(TGF-β1)和纤维连接蛋白(FN)表达的影响。方法:将原代培养的第3代RPMCs随机分为6组:对照组、1.5%葡萄糖组、2.5%葡萄糖组、单纯虫草组(10mg/ml)、1.5%葡萄糖+10mg/ml虫草组、2.5%葡萄糖+10mg/ml虫草组。同步培养72h后,以RTPCR法检测各组细胞TGF-β1和FN mRNA表达情况;ELISA法检测细胞上清液中TGF-β1和FN的蛋白质水平。结果:高糖作用下RPMC的TGF-β1、FN mRNA和蛋白表达水平均明显高于对照组(P<0.05),且增高的程度与葡萄糖浓度呈依赖关系。与相应浓度的高糖组相比,经虫草菌液干预后RPMC的TGF-β1、FN mRNA和蛋白表达水平均显著降低(P<0.05)。结论:虫草菌液可下调高糖作用下RPMCs的TGF-β1和FN的表达,具有抗腹膜纤维化的作用。

Objective：To investigate the effect of Hirsutella sinensis（HS） on the expression of transforming growth factor-β1（TGF-β1） and fibronectin（FN） in rat peritoneal mesothelial cells（RPMCs） cultured in high glucose concentration in vitro.Methods：The third generation RPMCs from primary culture were divided randomly into 6 groups：control group,1.5% glucose group,2.5% glucose group,HS（10 mg/ml） group,1.5% glucose＋HS（10 mg/ml） group,2.5% glucose＋HS（10 mg/ml） group.After 72 h,the mRNA and protein expression of TGF-β1 and FN were determined by RT-PCR and ELISA respectively.Results：The mRNA and protein expression of TGF-β1 and FN increased significantly in RPMCs cultured with high glucose,compared with control group（P0.05）,and accompanying with the increased concentration of glucose.As compared with high glucose group（1.5% or 2.5% glucose）,the mRNA and protein expression of TGF-β1 and FN decreased significantly in high glucose with Hirsutella sinensis （10 mg/ml） group（P0.05）.Conclusion：Hirsutella sinensis reduces the expression of TGF-β1 and FN induced by high glucose in RPMCs,and has the inhibited effect on peritoneal fibrosis.