抗肿瘤因子内皮抑素基因的克隆及表达

CLONING AND EXPRESSION OF AN ANITUMOR MOLECULE HUMAN ENDOSTATIN

为了克隆抗肿瘤因子内皮抑素基因并表达内皮抑素蛋白，从人胎盘组织中分离总ＲＮＡ， 用ＲＴ ＰＣＲ 法扩增出５７０ ｂｐ 的ＤＮＡ 片段，并成功地克隆到ｐＵＣ１８ 质粒载体中，经序列测定证实为内皮抑素基因，用Ｘｐｒｅｓｓ 系统体外表达出内皮抑素蛋白并纯化成功．

To clone and express human endostatin, total RNA was isolated from human placenta tissue and endostatin gene was amplified by RT PCR. Then, the 570 bp endostatin gene fragment was cloned into pUC18 vector for sequencing. It was expressed and purified by Xpress system. The success of cloning and expression of human endostatin gene will provide tool for high level expression of it and further clinical application as antitumor drug.