多指标综合评价优选大血藤酚酸类化合物的提取工艺

Multi-index Evaluation of Extraction of Phenolic Compounds from Sargentodoxa cuneata

目的:筛选出大血藤酚酸类化学成分的最佳提取工艺。方法:首先建立HPLC测定大血藤中没食子酸、原儿茶酸、绿原酸方法,采用正交试验设计,以提取时间、甲醇体积分数、溶媒用量、提取次数为因素考察大血藤回流提取方法,以没食子酸、原儿茶酸、绿原酸为评价指标,确定最佳提取条件。结果:优选工艺为用10倍量80%甲醇提取3次,每次30 min。结论:采用多指标综合评价法优化大血藤酚酸类化学成分提取工艺合理、可行。

Objective：To screen out the best extraction technique of chemical composition of phenolic acids from cuneata.Method： The HPLC method for determination of gallic acid,protocatechuic acid and chlorogenic acid in Sargentodoxa cuneata was firstly established.The orthogonal experimental design was used to study the optimum extraction with extracting time,ethanol concentration,solvent usage,extraction times as factors,gallic acid,protocatechuic acid,chlorogenic acid as the evaluation index to determine the best extraction technology.Result： the optimum extraction technology was 80% ethanol concentration,10 times of solvent usage,extracted for 30min,3 times of extraction.Conclusion： A multi-index evaluation method can optimize the extraction process conditions of chemical composition of phenolic acids from S.cuneata and provide a reference for separation and purification of total phenolic acids from S.cuneata.