调肝方药对愤怒、郁怒模型大鼠下丘脑5-HTR2C基因表达的影响

Effects of Tiaogan Fangyao on Expression of 5-HTR2C in Hypothalamus of Rats with Anger-in/out Constitution

目的:通过研究愤怒和郁怒模型大鼠下丘脑5-HTR2C基因的表达,探讨愤怒和郁怒的微观发生机制及中药经前平颗粒、经前舒颗粒的作用靶点。方法:居住入侵法制备愤怒和郁怒证模型大鼠,经前平颗粒、经前舒颗粒干预,均10 g·kg-1,ig,分别为正常组、模型组、经前舒组、经前平组;运用半定量RT-PCR和Western blot方法检测大鼠下丘脑区5-HTR2C mRNA及其蛋白表达水平。结果:与正常组相比较,愤怒组和郁怒组大鼠下丘脑中5-HTR2C mRNA和蛋白相对水平均极显著性降低(P<0.01);愤怒组大鼠的相对表达水平改变明显强于郁怒组大鼠;用药后,与模型组相比较,5-HTR2C mRNA均得到显著性改善,但与正常组相比仍极显著性下降(P<0.01),而蛋白改变均得到极显著性改善,与正常组相比无统计学差异。结论:模型组大鼠下丘脑与5-HTR2C基因表达极显著降低,调肝方药干预可显著上调模型大鼠下丘脑5-HTR2C mRNA和蛋白表达,说明5-HTR2C基因与愤怒和郁怒证相关,并且可能是经前平颗粒和经前舒颗粒治疗该证的作用机制之一。

Objective：To investigate the association of the pathogenesis and drug action target of Jingqianping and Jingqianshu granule with the expression level of 5-HTR2C in hypothalamus of rats with anger-in and anger-out constitution.Method：The model rats were prepared by the methods of residential intrusion,and treated with Jingqianping or Jingqianshu granule.Then we gained control groups,model group and treated groups.The expression of 5-HTR2C in the hypothalamus of the models was detected by RT-PCR and western blotting.Result：The levels of 5-HTR2C mRNA and protein in the hypothalamus of models decreased significantly as compared to the controls,and the levels of the anger-out-model group were higher than the anger-in group.And the levels of 5-HTR2C mRNA and protein in groups treated with Jingqianping or Jingqianshu granule increased significant as compared to the models.There were significantly difference for mRNA between the treated and the control rats.There were no significant difference for protein between the treated and the control rats.Conclusion：The expression of 5-HTR2C in the hypothalamus of the two model group decreased significantly compared to the controls,and then the protein recovered to the control level and the mRNA was adjusted after treated with Jingqianping or Jingqianshu granule.So,the result demonstrates that the association of the expression of 5-HTR2C maybe exist with anger-out and anger-in.And the gene could be one of the action target of Jingqianping and Jingqianshu granule.