青黛镇痛、抗炎药效学研究

Pharmacokinetics Research on Anti-Inflammatory Effect and Analgesic Effect of Indigo Naturalis

目的:观察不同给药方式下青黛的镇痛、抗炎作用。方法:分别采用ig和外涂两种给药途径,用药4 d。动物分为空白对照组,阳性药组以及青黛治疗组(600,300,150 mg·kg-1 ig 3个剂量;外涂青黛给药剂量为小鼠400 mg/只、大鼠500 mg/只)。以热板法和小鼠扭体法观察青黛的镇痛作用;以二甲苯致小鼠耳廓肿胀法、大鼠棉球肉芽肿法观察青黛的抗炎作用。结果:ig给药,青黛对热板所致的小鼠疼痛可使痛阈提高达47%,醋酸致小鼠疼痛的扭体减少达40%,与空白组比较差异显著(P<0.05或P<0.01);对急性炎症模型耳廓肿胀抑制率为48%,与空白组比较抗炎作用显著(P<0.05),对亚急性炎症模型作用不明显;外涂给药,对热板法致的小鼠疼痛有显著镇痛作用(P<0.01),对醋酸所致的小鼠扭体反应影响不明显,对急性、亚急性炎症模型均有明显抗炎作用(P<0.01)。结论:结果提示青黛内服、外用均有一定的镇痛、抗炎作用;不同给药途径对于不同疼痛、炎症模型的影响表现出一定的差异性。

Objective：To investigate the analgesic and anti-inflammatory effects of Indigo Naturalis by different administrations.Method： Two administration ways（ig and external application）were applied respectively for 4 days.The experimental animals were randomly divided into different groups：blank control group,positive control group and Indigo Naturalis therapy groups（150,300,600 mg·kg-1 by ig;400 mg for a mice and 500 mg for a rat by external application）.Analgesic effects of Indigo Naturalis were investigated by hot plate test and acetic acid writhing test in mice;anti-inflammatory effects of Indigo Naturalis were investigated by xylene-induced aruical swelling in mice and granuloma induced by cotton ball implantation in rats.Result： By ig,Indigo Naturalis decresed the pain threshold in mice caused by hot plate test to 47% and acetic acid writhing test to 40%,shown a strong difference,compared with blank control group（P0.05,P0.01）.The inhibition rate of aruical swelling was 48% on the acute inflammation model,shown a striking anti-inflammatory effect,compared with blank control group（P0.05）;but there was no great effect on the sub-acute inflammation model.By external application,Indigo Naturalis had a good analgesic effect to the pain in mice caused by hot plate（P0.01）,but did not had a good effect in the acetic acid writhing test.However,it showed good effects on both acute and subacute inflammation models（P0.01）.Conclusion： The results show that Indigo Naturalis have certain analgesic and anti-inflammatory effects by different administrations.But there are several differences in the analgesic and anti-inflammatory effects due to the different ways of administration.