3种天然化合物联合对四氯化碳诱导的大鼠肝纤维化的影响

Effect of Three Natural Medicine Compounds on Liver Fibrosis Induced by Carbon Tetrachloride in Rats

目的:研究玉郎伞多糖(YLS)、玉郎伞黄酮(YF)与六月青多糖(LYQPS)联合对四氯化碳(carbon tetrachloride,CCl4)所致大鼠肝纤维化的干预作用及机制。方法:Wistar大鼠随机分成两组,模型组大鼠采用50%CCl4花生油溶液2mL·kg-1ig造模,共7周,正常组用生理盐水代替。将病理检查确认肝纤维化已形成的Wistar大鼠40只,随机分成4组,分别给予相应药物进行干预,每日1次,连续4周。末次给药24h后处死大鼠,采集肝组织,检测大鼠肝组织中羟脯氨酸(Hyp)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量,采用HE染色观察肝组织纤维化程度。结果:联合用药高剂量(540mg·kg-1)组中MDA(10.43±0.95)nmol·mg-1(P<0.01)和Hyp(0.26±0.05)μg·mg-1(P<0.05)含量明显下降,SOD(39.19±6.00)U·mg-1(P<0.05)活力显著升高;低剂量(270mg·kg-1)组有一定的保护肝脏组织的作用。各药物组肝组织细胞纤维化程度均有所减轻。结论:YLS,YF,YQPS联合用药可减轻CCl4诱导的肝纤维化。

Objective：To study the effect of the compounds of Yulangsan polyose,Yulangsan flavones and Liuyueqing polyose against CCl4-induced liver fibrosis in rats and its mechanism.Method： Wistar rats were divided into two groups randomly：hepatic fibrosis model group and normal control group.The model group of rats with hepatic fibrosis was established by 2 mL·kg-1 intragastric administration（ig）of 50%CCl4 for 7 weeks.The rats in the normal control group were given normal saline（NS）.Forty Wistar rats with hepatic fibrosis confirmed by the pathological inspection were divided into 4 groups randomly.All rats were treated with drugs or NS for four consecutive weeks by ig,once daily.Twenty-four hours after the last administration of drugs,all rats were killed,and the hepatic tissue were taken.The contents of Hyp,SOD,MDA in hepatic tissue were measured,the extent of liver fibrosis was observed by HE staining.Result： The contents of MDA（10.43±0.95） nmol·mg -1（P0.01）,Hyp（0.26±0.05） μg·mg-1（P0.05）were decreased significantly and the contents of SOD（39.19±6.00） U·mg-1（P0.05）were increased significantly in high dose group.All drug groups could relieve liver Fibrosis.Conclusion： The compounds of Yulangsan polyose,Yulangsan flavones,Liuyueqing polyose can relieve liver fibrosis induced by CCl4.