多棘蜈蚣与少棘蜈蚣抗肿瘤作用研究

Tumor-inhibitory Effect of Prickly Centipede and Less Thorn Centipede

目的:探讨两种蜈蚣的抗肿瘤作用。方法:以MTT法测定两种蜈蚣的含药血清对人肝癌细胞系Bel-7402生长的抑制作用;体内试验将小鼠接种S180实体瘤后检测两种蜈蚣对小鼠S180实体瘤生长的抑制作用。结果:两种蜈蚣均可明显抑制肝癌细胞株Bel-7402的增殖;多棘蜈蚣和少棘蜈蚣0.2,0.4,0.6 g.kg-1对小鼠S180实体型肿瘤均有明显抑制作用(P<0.01),抑瘤率分别为60.95%,63.68%,69.01%和48.52%,50.18%,50.83%。结论:两种蜈蚣均具有较强的抗肿瘤作用,无论体内和体外试验,多棘蜈蚣均有优于少棘蜈蚣的趋势。

Objective：To investigate the effects two kinds of centipede on anti-tumor in vivo.Method：The effect of the two kinds of medicine contained in serum for growth inhibition of human liver cell Bel-7402 was evaluated by MTT.Inhitition of inoculated S180 tumor in mice was used to compare the two kinds of centipede.Result： There were different inhibition for liver cell proliferation of Bel-7402 of the two kinds of centipede;the inhibition rates of S180 tumors in mice were 60.95%,63.68%,69.01% and 48.52%,50.18%,50.83% by the prickly and less thorn of centipede（0.2,0.4,0.6 g·kg-1）.There were obvious inhibition to grow of S180 tumor in mice（P0.01）.Conclusion： Two kinds of centipede have strong antitumor effect.Prickly centipede shows a tendency of stronger effect than that of the less thorn centipede.