补骨脂对去卵巢大鼠雌激素样作用研究

Estrogenic Effect of Psoraleae Fructus in Ovariectomized Rats

目的:观察补骨脂对去卵巢大鼠的雌激素样作用。方法:SD雌性大鼠行双侧卵巢摘除术(假手术组只切除卵巢附近脂肪),随机分为去卵巢模型组、补骨脂组(0.59 g.kg)、补佳乐组(0.8 g×10-4 g·kg-1)和假手术组,ig 4周后分别观察大鼠肛温、子宫和肾上腺系数,ELISA法检测血清雌二醇(E2)、黄体生成素(LH)、促卵泡生成素(FSH)水平。结果:模型组大鼠与假手术组比较肛温升高,子宫和肾上腺萎缩,血清中E2水平下降,LH,FSH水平升高。与模型组比较,补骨脂组、补佳乐组大鼠的肛温明显降低(P<0.01),子宫和肾上腺系数明显提高(P<0.05,P<0.01),血中E2浓度明显升高,LH,FSH水平明显降低(P<0.01)。结论:补骨脂能降低去卵巢大鼠的肛温,增加子宫和肾上腺系数,升高血中E2水平并降低LH,FSH水平,表明补骨脂对去卵巢大鼠有雌激素样作用。

Objective：To investigate the estrogenic effects of Psoraleae Fructus in ovariectomized rats.Method： Female SD rats were ovariectomized（ovx）and randomly assigned to four groups：ovariectomized without treatment,sham operated,ovx rats treated with 0.08 mg·kg-1 progynova and ovx rats treated with Psoraleae Fructus at dose of 0.59 g·kg-1 for four weeks.The rectal temperature,wet weight of uterus and adrenal gland were observed,levels of estradiol（E2）,luteinizing hormone（LH） and follicle stimulating hormone（FSH） in serum were detected by ELISA.Result： Compared with untreated OVX controls,both Psoraleae Fructus and progynova treatments showed significant and clear estrogenic activity as indicated by increased weight of uterus and adrenal gland,and the reduction in rectal temperature（P0.01）.Significant increased in serum E2 and decreased in serum LH and FSH were found compared with the untreated OVX controls（P0.01）.Conclusion： Psoraleae Fructus has estrogenic activity in ovariectomized rats.