钙调素特异性拮抗剂(TFP)对大鼠着床的影响

The Effects of Calmodulin Antagonist Distinctive TFP on Implantation of RATS

在大鼠孕４ ｄ 宫腔内一次性注入钙调素特异性拮抗剂—三氟拉嗪（ＴＦＰ），每侧子宫角０．０５ｍ Ｌ（内含０．２５ ｍ ｇ），具有显著的抗着床作用，抗着床率高达１００％ ，而注入等容量生理盐水的对照组为０％ ．应用放射免疫测定技术发现，该剂量的ＴＦＰ能显著降低血清中孕酮（Ｐ）的含量，但对雌二醇（Ｅ２）则无明显影响，也不影响卵巢重及黄体数量．宫内注入ＴＦＰ能抑制假孕致蜕膜化反应，抑制程度与药量有关．结果表明，钙调素（ＣａＭ）在着床过程中起重要作用，此作用与血清孕酮水平有关，ＣａＭ 参与子宫内膜蜕膜化过程，而与血清中雌激素浓度无直接关系．

An injection of CaMA distinctive TFP (Trifluoperazine) administered to 4 days pregnant rats in the uterine cavity by the dose of 0.25 mg/0.05 mL into the uterine corner of each side can yield notable effects on anti-implantation. This anti-implantation rate reaches up to 100% . Whereas the rate is 0% among the control group that take same capacity of injection of physiological (normal) saline in the uterus. It is discovered through radio-immune determining techniques that this dosage of TFP for anti-implantation can markedly reduce the Progesterone content in blood serum, but has no distinct effect on Estradiol and won't either affect the ovary weight or the quantity of corpus luteum. The injection of TFP in the uterus can inhibit the pseudopregnancy-deciduomata reaction in a degree related to the dosage. These results show that in the process of implantation CaM plays an important part by participating in the deciduomata process of the uterine membrane,and that the effects are connected with the Progesterone level in serum instead of having any direct relation with the concentration of estrogenic hormone in serum.