罗哌卡因预处理对其诱导ND7／23细胞毒性的影响

Effect of ropivacaine preconditioning on toxicity of ropivacaine to ND7/23 cells

目的评价罗哌卡因预处理对其诱导ND7／23细胞毒性的影响。方法正常培养ND7／23细胞，选择指数生长期的细胞，将其接种于96孔板上继续培养24h，浓度为1×10^6个／ml。采用随机数字表法，将细胞随机分为3组，每组3孔：对照组（C组）不给予任何处理；罗哌卡因组（R组）加入1％罗哌卡因100μl作用1h；罗哌卡因预处理组（RP组）先加入0．02％罗哌卡因100μl作用15min，洗脱后，再加入1％罗哌卡因100μl作用1h。采用CCK-8染色法检测细胞活力，采用Armexin-V／PI法检测细胞凋亡。结果与C组比较，R组和RP组细胞活力降低，早期凋亡率和晚期凋亡率升高（P〈0．05）；与R组比较，RP组细胞活力升高，早期凋亡率和晚期凋亡率降低（P〈0．05）。结论罗哌卡因预处理可通过抑制细胞凋亡减轻其对ND7／23细胞的毒性作用。

Objective To investigate the effect of ropivacaine preconditioning on the toxicity of ropivacaine to ND7/23 cells. Methods ND7/23 cells were cultured in DMEM culture medium at 37 ℃ in 5 % CO2 incubator for 24 h at a concentration of 1 × 10^6/ml. The ceils were randomly divided into 3 groups （ n = 3 each） ： control group （group C）, repivacaine group （group R） and ropivacaine preconditioning group （group RP）. In group R, the cells were exposed to 1% ropivacaine 100 μl and incubated for 1 h. In group RP, the cells were exposed to 0.02% repivacaine 100μl for 15 min, after repivacaine was washed out, 1% ropivacaine 100μl was then added and the cells were incubated for 1 h. The cell viability was measured using CCK-8 assay and apoptosis using Annexin-V/PI staining. Results Compared with group C, the cell viability was significantly decreased, while the early .apoptotic rate and late apoptotic rate were significantly increased in groups R and RP （P 〈 0.05）. Compared with group R, the cell viability was significantly increased, while the early apoptotic rate and late apoptotic rate were significantly decreased in group RP （ P 〈 0.05）. Conclusion Ropivacaine preconditioning can protect NDT/ 23 cells from bupivacaine-induced cytotoxicity through inhibiting apoptosis.