辛伐他汀对脓毒症大鼠内皮细胞功能的影响

Effect of simvastatin on endothelial cell function in a rat model of sepsis

目的探讨辛伐他汀对脓毒症大鼠内皮细胞功能的影响。方法清洁级雌性Wistar大鼠96只，体重200～250g，月龄4月。采用随机数字表法，将大鼠随机分为3组（n=32）：假手术组（Ⅰ组）、脓毒症组（Ⅱ组）和辛伐他汀组（Ⅲ组）。选模前，Ⅲ组经胃管给予辛伐他汀20mg／kg，1次／d，连续2周，Ⅰ组和Ⅱ组用等容量生理盐水替代。Ⅱ组和Ⅲ组采用盲肠结扎穿孔法制备脓毒症模型。于术后3、6、24及48h时分别取8只大鼠，采集颈动脉血样，测定血常规，计数WBC，用ELISA法检测血清E．选择素浓度。结果与Ⅰ组比较，Ⅱ组和Ⅲ组WBC计数和血清E-选择素浓度升高，术后6h时达峰值（P〈0．05）；与Ⅱ组比较，Ⅲ组WBC计数和血清E-选择素浓度降低（P〈0．05）。结论辛伐他汀治疗脓毒症的作用机制与保护内皮细胞功能有关。

Objective To investigate the effect of simvastatin on endothelial cell function in a rat model of sepsis. Methods Ninety-six pathogen-free female Wistar rats aged 4 months weighing 200-250 g were randomly divided into 3 groups （ n = 32 each） ： group sham operation （group Ⅰ ） ; group sepsis （group Ⅱ ）and group simvastatin ＋ sepsis（group Ⅲ ）. Sepsis was induced by cecal ligation and puncture （CLP）. In group Ⅲ simvastatin 20 mg/kg was given via a gastric tube once a day for 2 weeks. Blood samples were taken from carotid artery at 3, 6, 24 and 48 h （ n = 8 at each time point） for WBC count and measurement of serum E-seleetin concentration （by ELISA） . Results CLP significantly increased WBC count and serum E-seleetin concentration in group Ⅱ as compared with group Ⅰ . The peak values were reached at 6 h after operation. Simvastatin pretreatment attenuated the sepsis-induced increase in WBC count and serum E-selectin concentration in group Ⅲ. Conclusion Protection of endothelial cell function is involved in the mechanism of treatment of sepsis with simvastatin.