摘要
目的探讨辛伐他汀对脓毒症大鼠内皮细胞功能的影响。方法清洁级雌性Wistar大鼠96只,体重200~250g,月龄4月。采用随机数字表法,将大鼠随机分为3组(n=32):假手术组(Ⅰ组)、脓毒症组(Ⅱ组)和辛伐他汀组(Ⅲ组)。选模前,Ⅲ组经胃管给予辛伐他汀20mg/kg,1次/d,连续2周,Ⅰ组和Ⅱ组用等容量生理盐水替代。Ⅱ组和Ⅲ组采用盲肠结扎穿孔法制备脓毒症模型。于术后3、6、24及48h时分别取8只大鼠,采集颈动脉血样,测定血常规,计数WBC,用ELISA法检测血清E.选择素浓度。结果与Ⅰ组比较,Ⅱ组和Ⅲ组WBC计数和血清E-选择素浓度升高,术后6h时达峰值(P〈0.05);与Ⅱ组比较,Ⅲ组WBC计数和血清E-选择素浓度降低(P〈0.05)。结论辛伐他汀治疗脓毒症的作用机制与保护内皮细胞功能有关。
Objective To investigate the effect of simvastatin on endothelial cell function in a rat model of sepsis. Methods Ninety-six pathogen-free female Wistar rats aged 4 months weighing 200-250 g were randomly divided into 3 groups ( n = 32 each) : group sham operation (group Ⅰ ) ; group sepsis (group Ⅱ )and group simvastatin + sepsis(group Ⅲ ). Sepsis was induced by cecal ligation and puncture (CLP). In group Ⅲ simvastatin 20 mg/kg was given via a gastric tube once a day for 2 weeks. Blood samples were taken from carotid artery at 3, 6, 24 and 48 h ( n = 8 at each time point) for WBC count and measurement of serum E-seleetin concentration (by ELISA) . Results CLP significantly increased WBC count and serum E-seleetin concentration in group Ⅱ as compared with group Ⅰ . The peak values were reached at 6 h after operation. Simvastatin pretreatment attenuated the sepsis-induced increase in WBC count and serum E-selectin concentration in group Ⅲ. Conclusion Protection of endothelial cell function is involved in the mechanism of treatment of sepsis with simvastatin.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2011年第4期500-502,共3页
Chinese Journal of Anesthesiology