erbB4和PTEN在皮肤鳞状细胞癌组织中的表达

Expression of erbB4 and PTEN in cutaneous squamous cell carcinoma tissue

目的观察并探讨erbB4和PTEN在皮肤鳞状细胞癌（鳞癌）组织中的蛋白表达及其临床意义。方法采用免疫组化Elivision法，检测52例皮肤鳞癌（其中11例伴有淋巴结转移，41例无转移）、10例正常人皮肤标本中erbB4和PTEN蛋白表达。结果皮肤鳞癌组中39例erbB4蛋白呈阳性表达，阳性率为75％；对照组中仅1例阳性表达，两组阳性率比较，X^2=12．77，P〈0．01；皮肤鳞癌患者中在有淋巴结转移组erbB4蛋白阳性表达率（100％）明显高于无淋巴结转移组（68．29％），两组阳性率比较，P〈0．05。PTEN蛋白在皮肤鳞癌组中25例阳性表达，阳性率为48．08％，对照组10例均为阳性表达，两组阳性率比较，X^2=9．20，P〈0．01；在鳞癌高分化组及中低分化组中的阳性率分别为78．57％、36．84％，两组差异有统计学意义（P〈0．05）；有淋巴结转移组PTEN阳性率（9．09％）明显低于无转移组（58．54％）（P〈0．01）。皮肤鳞癌erbB4蛋白与PTEN蛋白表达呈负相关（r=-0．42，P〈0．01）。结论erbB4与FINN可能参与了皮肤鳞癌的发生、恶性进展及转移。

Objective To investigate the expression of erbB4 and PTEN in cutaneous squamous cell carcinoma （SCC） tissue and their significance. Methods Immunohistochemical Elivision method was used to measure the protein expression of erbB4 and PTEN in tissue specimens from 52 patients with SCC （11 cases with and 41 cases without lymph node metastasis） and 10 normal human controls. Results The positivity rate of erbB4 was significantly higher in the patients with SCC than in the normal human controls （75% vs. 10%,X^2= 12.77, P 〈 0.01）, and in the patients with lymph node metastasis than in those without （100% vs. 68.29%, P 〈 0.05 ）. Significant differences were also observed in the expression rate of PTEN between the patients with SCC and the normal controls （48.08% vs. 100%,X^2 = 9.20, P 〈 0.01 ）, between the patients with well-differentiated SCC and those with poorly differentiated SCC （78.57% vs. 36.84%, P 〈 0.05）, and between the patients with lymph node metastasis and those without （9.09% vs. 58.54%, P 〈 0.01 ）. The expression of erbB4 was negatively correlated with that of PTEN （r = -0.42, P 〈 0.01 ）. Conclusion The disturbance in mutual regulation between erbB4 and PTEN seems to be involved in the initiation, progression and metastasis of SCC.