负向调控调节性T细胞对小鼠动脉粥样硬化形成的影响

Roles of Naturally Regulatory T Cells Regulated Negatively in Experimental Atherosclerosis of ApoE^(-/-) Mice

目的通过干扰FOXP3基因来研究负向调控天然调节性T细胞对ApoE基因敲除小鼠动脉粥样硬化的影响。方法构建FOXP3-siRNA慢病毒载体和获取Foxp3hight+CD4+CD25+Treg细胞分别通过尾静脉注入不同组小鼠体内;利用流式细胞仪检测小鼠体内CD4+CD25+Treg细胞数目;利用ELISA法检测各组脾细胞炎性因子浓度;取小鼠升主动脉动脉行病理分析其粥样斑块大小。结果注入FOXP3-siRNA慢病毒载体的小鼠CD4+CD25+Treg细胞数目减少,动脉粥样斑块面积显著增大。而注入Foxp3hight++CD4+CD25+Treg细胞则相反,小鼠CD4+CD25+Treg细胞数目增加,动脉粥样斑块面积减小。结论负向调控天然调节性T细胞能促进动脉粥样硬化的形成。

Aim The aim of this study was to examine the effect of naturally regulatory T cells on atherosclerosis plaque in apoliprotein（apo）E-/- Mice,through negative regulating FOXP3 gene. Methods Lentivirus-mediated（siRNA） was used and Foxp3high＋CD4＋ CD25＋ Treg cells adoptive transfer assays in high fat diet ApoE-/- Mice were done.Its number was identified by FACS.Inflammatory cytokines were determined by ELISA and the area of atherosclerosis plaque was analyzed. Result We found that the number and function of Foxp3＋CD4＋CD25＋ Treg cells in mice injected with Foxp3high＋CD4＋ CD25＋ Treg cells was significantly high compared with those of mice injected with FOXP3-siRNA lentivirus. Conclusion Naturally regulatory T cells regulated negatively can promote significantly the progression of atherosclerosis plaque in ApoE-/- Mice.