瑞舒伐他汀对兔动脉内皮损伤后转化生长因子β1表达的影响

Effect of Rosuvastatin on Expression of Transforming Growth Factor β1 in Dorsal Aorta of Rabbits After Balloon Injury

目的探讨瑞舒伐他汀对兔腹主动脉球囊损伤后转化生长因子β1表达的影响。方法新西兰兔20只随机分为瑞舒伐他汀组和模型组,两组均应用球囊损伤腹主动脉方法建立兔血管内皮损伤模型,术后6周行腹主动脉造影后采用免疫组织化学法和逆转录聚合酶链反应技术对兔腹主动脉壁转化生长因子β1表达的情况进行观察。结果瑞舒伐他汀组血管狭窄程度(31.41%±7.08%)、血管狭窄段最小内径(1.74±0.25 mm)明显优于模型组(分别为55.25%±10.23%和1.32±0.33 mm),两组比较差异有统计学意义(P<0.05);瑞舒伐他汀组转化生长因子β1平均吸光度(0.41±0.12)、阳性细胞面积(31.37%±4.42%)均低于模型组(0.57±0.17和40.58%±7.45%,P<0.05),损伤血管中转化生长因子β1 mRNA表达(22.37±4.42)较模型组(42.47±5.41)明显减弱(P<0.05)。结论瑞舒伐他汀对内皮损伤后的血管病变有保护作用,其机制可能是部分通过下调转化生长因子β1的表达,减轻血管球囊损伤后再狭窄。

Aim To evaluate the effect of rosuvastatin on restenosis and the expression of transforming growth factor β1（TGF-β1） in abdominal aorta of rabbits after balloon injury. Methods Two groups of 10 male New Zealand rabbits received either rosuvastatin or no rosuvastatin after balloon injury.We did abdominal aorta angiography and observed the expression of TGF-β1 with immunohistochemistry and reverse transcription polymerase chain reaction（RT-PCR） six weeks later. Results The average stenosis degree（31.41%±7.08%） and the smallest inner diameters（1.74±0.25 mm） were better in rosuvastatin-treated group than that of the model group（55.25%±10.23% and 1.32±0.33）.The average absorbency and the area of positive of TGF-β1 were dramatically lower in rosuvastatin-treated group than that of the model group（0.41±0.12 vs 0.57±0.17,31.37%±4.42% vs 40.58%±7.45%）;The quantity of amplification products（22.37±4.42） was lower than that of the model group（42.47±5.41）,there were significant differences between the two groups（P0.05）. Conclusion Rosuvastatin can improve the restenosis and decrease the expression of TGF-β1 in rabbits after balloon injury.