人外周血、脐血、脂肪组织来源的内皮祖细胞部分生物学特性比较

Study on Some Biological Characteristics of Endothelial Progenitor Cells from Human Peripheral Blood,Umbilical Cord Blood and Adipose Tissue

目的研究人外周血、脐血和脂肪组织三种不同来源内皮祖细胞部分生物学特性异同,探讨其可能不同的适应范围和方式及方法,进而为提高内皮祖细胞的应用效率提供依据。方法采用密度梯度离心法分离人外周血、脐血单个核细胞,胰蛋白酶消化提取脂肪组织干细胞。所采集细胞诱导分化,观察细胞形态,对培养第7天的细胞进行鉴定。采用MTT法检测细胞增殖,Boyden小室检测细胞迁移能力,贴壁法检测细胞黏附功能以及血管生成试剂盒检测细胞体外成血管能力。结果诱导分化细胞均证实为内皮祖细胞。脐血源性内皮祖细胞增殖、迁移、黏附、成血管能力最强(P<0.05)。脂肪组织来源内皮祖细胞增殖与成血管能力方面较外周血来源内皮祖细胞强(P<0.05)。结论人脐血内皮祖细胞可能更适用于同种异体干细胞移植,脂肪组织来源内皮祖细胞可能在自体干细胞移植治疗缺血性疾病方面有潜在应用前景。

Aim To investigate some biological characteristics of similarities and differences of endothelial progenitor cells from human peripheral blood,umbilical cord blood and adipose tissue and provide the evidences for efficacious applications of endothelial progenitor cells on different adaptive scopes,ways and methods. Methods Mononuclear cells were isolated from umbilical cord blood and adult peripheral blood using a Ficoll-Hypaque density gradient centrifugation.Adipose tissue-derived stem cells were isolated from the adipose tissue by trypsin digestion.The collected cells were cultured.The cell morphology was observed and the cultured cells were identified at the seventh day.The proliferation and migration of endothelial progenitor cells were measured by MTT and Boyden chamber assay.The adhesion function and vasculogenesis activity in vitro of endothelial progenitor cells were analyzed by adhesion assay and in vitro vasculogenesis kit. Results All differentiation cells were identified as endothelial progenitor cells.Endothelial progenitor cells from umbilical cord blood were revealed superior in proliferation,migration,adhesion and vasculogenesis activity（P0.05）.The proliferation and vasculogenesis activity of endothelial progenitor cells from adipose tissue were more powerful than those of endothelial progenitor cells from adult peripheral blood（P0.05）. Conclusion Endothelial progenitor cells from umbilical cord blood may be more suitable for allogeneic stem cell transplantation.Endothelial progenitor cellsfrom adipose tissue may exhibit potential applications in autologous stem cell transplantation for ischemic disease.