期刊文献+

马铃薯茎尖培养一步成苗技术研究 被引量:5

Study on Technique of One-step Seedling Culture for Shoot Tip of Potato
下载PDF
导出
摘要 选取优质马铃薯顶芽作外植体,系统研究了马铃薯茎尖脱毒、壮苗及一步成苗生产技术。结果表明:带菌少的外植体,污染率低,茎尖成活率高;在MS培养基中加入0.2~0.8 mg/L CCC(矮壮素)对脱毒马铃薯试管苗兼有促进增殖、健壮,提高增殖系数,以及促发继代后生根的多重效果;而且试管苗移栽后缓苗期短,生长旺盛。采用一步成苗技术大规模生产,可以简化组培程序,缩短育苗周期,降低生产成本。 The quality apical buds of potato were selected as explants to study the techniques of shoot tip detoxification,vigorous tube-seedlings and one-step seedling culture for potato.The results showed that the less bacterium the explants carried,the lower the contamination rate and the higher the shoot tip survival rate was;adding 0.2~0.8 mg/L of CCC to the MS culture medium had multiple effects for virus-free potato tube-seedlings,which included promoting proliferation and vigorous growth of seedlings,enhancing proliferation coefficient and rooting after subculture;moreover,the tube-seedlings had short temporary planting period and vigorous growth after transplanting.Applying technique of one-step seedling culture to large scale production can simplify process of tissue culture,shorten cycle of seedling breeding,and reducing cost of production.
出处 《湖南农业科学》 2011年第6期59-62,共4页 Hunan Agricultural Sciences
基金 脱毒马铃薯良种繁育项目(农计发[2007]49号)
关键词 脱毒马铃薯 茎尖培养 试管壮苗 一步成苗 virus-free potato shoot tip culture vigorous tube-seedlings one-step seedling culture
  • 相关文献

参考文献14

二级参考文献37

共引文献81

同被引文献46

引证文献5

二级引证文献23

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部