内蒙古地区西门塔尔牛亲子鉴定微卫星标记筛选

Identification of Microsatellite Markers for Simmental Paternity Testing

研究旨在筛选适合于我国肉牛亲子鉴定的微卫星标记,以内蒙古乌拉盖地区368头西门塔尔牛为实验材料,利用荧光引物PCR和ABI3730测序仪对12个微卫星标记进行基因型检测,应用Cervus3.0软件统计12个微卫星标记的遗传多态性。结果表明:其中4个微卫星标记扩增效率较低,被剔除;其他8个微卫星的多态性丰富,平均等位基因数为11.38,平均期望杂合度为0.6686,平均多态信息含量为0.6354,适合于我国肉牛亲子鉴定。

To identify the microsatellite markers suitable to paternity testing of Chinese beef population,in this study,12 microsatellites were selected and genotyped with fluorescent dye-labelled PCR primers（5＇-FAM or HEX） and ABI3730 DNA Sequencer in 368 Simmental of Mongolia ulgai.Cervus3.0 program was used to calculate the polymorphism of the 12 microsatellites.The results showed that the efficiency of PCR amplification was low in 4 microsatellites which were deleted.The other 8 microsatellites were highly polymorphic,the mean number alleles per locus was 11.38,the mean expected heterozygosity was 0.6686,the mean polymorphic information content was 0.6354.Such results showed that the 8 microsatellite markers can be applied in parentage testing of Chinese beef.