EMIT法与HPLC法测定人血浆中环孢素A浓度的比较研究

Determination of Plasma Concentrations of CsA by EMIT vs. HPLC

目的:比较酶放大免疫(EMIT)法和高效液相色谱(HPLC)法测定人血浆中环孢素A(CsA)浓度的区别与相关性。方法:收集肾移植患者服药后的稳态谷浓度血样20份,分别用EMIT法和HPLC法进行测定,考察2种测定方法的区别和相关程度。结果:HPLC法和EMIT法检测的CsA血药浓度差异有统计学意义(P<0.05),EMIT法测定血浆中CsA浓度较HPLC法高26.4ng·mL-1,通过Passing-Bablok回归分析2种方法具有良好的相关性(r=0.9940)。结论:EMIT法和HPLC法测定CsA血浆药物浓度结果差异具有统计学意义,在临床进行CsA药物浓度监测中应予以关注并作相应调整。

OBJECTIVE：To compare the difference and correlation of HPLC and enzyme-multiplied immunoassay test （EMIT） for the determination of blood concentration of CsA in human plasma. METHODS： A total of 20 different concentrations of clinical samples were collected and determined by HPLC and EMIT respectively. The difference and correlation of 2 kinds of determination methods were investigated. RESULTS： There were significant differences in blood concentrations of CsA determined by HPLC and EMIT（P0.05）. CsA concentration determined by EMIT were 26.4 ng·mL-1 more than by HPLC. But a satisfactory correlation was achieved between the two methods（r=0.994 0）. CONCLUSIONS： There is statistical significance in plasma concentration of CsA determined by EMIT and HPLC. We should pay attention to CsA monitoring by EMIT and HPLC and make some adjustment.