高等植物脂氧合酶活性测定方法的研究进展

Research Advances in the Assay Methods of the Lipoxygenase Activity in Higher Plants

本文系统归纳了高等植物脂氧合酶活性检测方法的研究进展。常规检测方法可分为两类:一类基于检测脂氧合酶催化反应产生氢过氧化物的速度,包括胡萝卜素脱色法、Fe(CNS)3显色法、标准分光光度法、2-硫代巴比妥酸显色法、碘化钾-淀粉法、二甲苯酚橙法;另一类基于检测反应中底物摄取氧的速度,包括量压法、氧电极法、亚甲基蓝染色法。此外还有酶联免疫法、超弱发光发射光谱法以及放射性同位素标记法。其中,标准分光光度法和氧电极法为脂氧合酶活性检测的标准方法。本文可为特定高等植物脂氧合酶活性的高效、准确测定提供参考。

The research advances in the assay methods of the lipoxygenase activity in higher plants were systematically summarized in this review.The normal assay methods involve two categories.One is based on the rate determination of the hydroperoxide produced in the lipoxygenase-catalyzed reactions,including carotene bleaching,Fe（CNS）3 coloration,standard spectrophotometric,2-thiobarbituric acid coloration,potassium iodide-starch and xylenol orange methods.The other is based on the determination of the oxygen-absorbing rate of the substrates in the lipoxygenase-catalyzed reactions,including manometric,oxygen electrode and methylene blue staining methods.Other assay method includes enzyme linked immunosorbent assay,superweak luminescence emission spectrum assay and radioisotopic labelling method.Among all of the above methods,the standard spectrophotometric and the oxygen electrode methods are both regarded as the standard ones.This paper could provide a reference for the efficient and accurate determination of the lipoxygenase activity in the specific higher plants.