摘要
以枸杞为材料,建立基因组DNA的有效提取方法,并对10个枸杞品种进行RAMP技术分析研究。结果表明,利用10对引物共扩增出87个条带,其中多态性条带53个;多态性条带比率33.3%～81.9%,10个枸杞品种间的遗传距离为0.1111～0.8333;研究表明,利用RAMP进行枸杞的品种DNA指纹分析是可行的。
To develop valid methods of DNA extraction for Lycium chinense for RAMP analysis and research. The result showed that： ten primers were applied to amplification. A total of 87 DNA bands were detected. 53 bands among which were polymorphic. The rate of polymorphic bands was 33.3%-81.9%. 10 Lycium chinense cultivars genetic distances were from 0.1111 to 0.8333. In the study, the RAMP markers can be used for the studies of fingerprint of Lycium chinense sensitively.
出处
《中国农学通报》
CSCD
北大核心
2011年第16期61-64,共4页
Chinese Agricultural Science Bulletin
基金
国家林业局行业公益项目"北方主要林木品种指纹库构建及分子鉴定技术研究"(201104039)
