基于AFLP标记技术的三翅槭与三角枫亲缘关系分析

Analysis on the Genetic Relationship of Acer trialum L.and Acer buergerianum Miq.by AFLP

为了从分子水平上揭示三翅槭与三角枫的亲缘关系,明确三翅槭在槭属中的分类地位,利用AFLP分子标记技术对三翅槭1份材料和三角枫4份材料进行亲缘关系检测,并采用UPGMA法进行聚类分析。结果表明:8对引物组合共获得202条谱带,其中99条带具有多态性,多态性比率为49.01%,说明AFLP标记技术适用于三翅槭和三角枫亲缘关系研究。聚类分析表明,在遗传相似系数为0.71时,可将三翅槭和三角枫划分成2个相互独立的类群,说明三翅槭与三角枫在分子水平上的分类与形态学上的分类一致,此外三翅槭与三角枫之间的遗传相似系数为0.71也表明它们之间的亲缘关系较近。通过AFLP分子标记,成功分析了三翅槭与三角枫亲缘关系,为证明三翅槭是槭属新种提供了分子证据。

In order to make clear the taxonomic status of Acer trialum L.in Acer genus,the genetic relationship between Acer trialum L.and Acer buergerianum Miq.was studied based on AFLP （amplified fragment length polymorphism） technique.A tree diagram was constructed using UPGMA method.The results showed that 202 bands were amplified with 8 primer pairs.Among them,99 bands were polymorphic （49.01%）.The result showed that AFLP could be used to analyze genetic relationship between Acer trialum L.and Acer buergerianum Miq..Cluster analysis showed that Acer trialum L.and Acer buergerianum Miq.were divided into 2 different groups,respectively.According to the results,Acer trialum L.was a new specie of the genus of Acer by AFLP molecular evidence.