3种植物无菌苗增殖与生根一体化技术研究

Studies on the Technology of Proliferation and Rooting Integration with Aseptic Seedlings in Three Species

在常规培养环境条件(温度(25±3)℃、湿度60%～70%、光强1500～2000lx,10～12h/天)下,以中华芦荟、驱蚊香草、三倍体毛白杨无菌芽或叶片为材料,研究不同培养基对其无菌苗增殖、生根的影响,探讨其一体化成苗技术。结果表明:中华芦荟无菌芽转接在MS+6-BA 2.0 mg/L+NAA 0.1mg/L+AC 0.1%培养基中50天,分化芽数平均达3.4个;生根率达100%,单株根数达6.1条,单株总根长达52.0cm,且根系粗长、黄白色。驱蚊香草无菌芽在MS+6-BA 3.0mg/L+NAA 0.2mg/L+AC 0.05%培养基中增殖速度最快,转接后45天分化芽数平均为10.2个;生根率为100%,生根苗叶片呈深绿色,生根质量好。三倍体毛白杨无菌叶片接种在MS+6-BA 0.5mg/L+KT 0.4mg/L+NAA 0.2mg/L+Ad 25.0mg/L+AC 0.05%培养基中55天,分化芽数平均达8.6个;生根率达92%,且根系较粗长、浅白色。

Under conventional culture conditions （（25±3）℃,relative humidity 60%-70%,1500-2000 lx for 10-12 h/d）,aseptic buds or leaves from the plants of Aloe vera var.chinensis,Pelargonium citrosum vanleenii and triploid Populus tomentosa were respectively used to research the technology of proliferation and rooting integration.The results showed that：after 50 days when aseptic buds of Aloe vera var.chinensis were transferred in MS ＋ 6-BA 2.0 mg/L＋ NAA 0.1 mg/L＋ AC 0.1%,the average number of differentiated buds reached 3.4 and the rooting rate reached 100% with 6.1 roots which were sturdy,long and yellow-white and 52.0 cm length of all roots per plant.After 45 days transferred in MS ＋ 6-BA 3.0 mg/L ＋ NAA 0.2 mg/L ＋ AC 0.05%,aseptic buds of Pelargonium citrosum vanleenii proliferated most fastest with the average number of differentiated buds reaching 10.2,and the rooting rate amounted to 100% with dark-green leaves.After 55 days when aseptic leaves of triploid Populus tomentosa were transferred in MS ＋ 6-BA 0.5 mg/L ＋ KT 0.4 mg/L ＋ NAA 0.2 mg/L ＋ Ad 25.0 mg/L ＋ AC 0.05%,differentiated buds arrived at an average of 8.6 and the rooting rate achieved 92% with more and longer,light-white roots.