蟾酥注射液对马立克氏病疫苗的保护作用

The Protective Action of Venenum Bufonis Injection on Marek's Disease Virus Vaccine

为了观察蟾酥注射液对鸡马立克氏病疫苗的调节作用,采用中药免疫药理学方法,选择不同浓度的蟾酥注射液与马立克氏病疫苗联合使用,作为试验组,免疫前及免疫后7日、14日和21日龄,通过静脉采集血液,采用MTT法,检测外周血T、B淋巴细胞增值率;采用显微镜计数法,计算血液中白细胞总数;采用鸡腹腔注射红细胞悬液,检测巨噬细胞吞噬力;采用黄嘌呤氧化酶法,测定血清中总的SOD活力;采用腹腔计数200个巨噬细胞所吞噬的红细胞数,计算吞噬百分率和吞噬指数的方法,检测巨噬细胞吞噬力;采用ELISA法,检测血清总抗体效价;采用胸腺、脾脏和法氏囊称重法,检测免疫器官指数。结果显示所有试验组中,蟾酥注射液均能够显著提高外周血T淋巴细胞的增值率;试验组中的高剂量组(0.25mg/mL)和中剂量组(0.1mg/mL),蟾酥注射液均能够显著提高血液中的白细胞总数;所有试验组中,蟾酥注射液均能够显著提高巨噬细胞的吞噬率和吞噬指数;试验组中的高剂量组和中剂量组,蟾酥注射液均能够显著提高SOD含量;所有试验组中,蟾酥注射液对马立克氏病疫苗抗体的产生均有不同程度的促进作用;所有试验组中,蟾酥注射液能够显著促进机体免疫器官的发育。说明蟾酥注射液能从特异性免疫、非特异性免疫、免疫细胞和免疫分子等方面增强免疫活性,研究结果显示蟾酥注射液是马立克氏病疫苗有效的免疫增强剂。

In the present study, researchers observed the regulating action of Venenum Bufonis injection solution on chicken Marek＇s disease virus vaccine. Methods： Researchers choose the traditional Chinese immunopharmacology method and combined different concentration Venenum Bufonis injection and Marek＇s disease virus vaccine as test groups. Before immunization and on the 7th, 14th and 21st day after immunization, researchers took blood samples through vein and choose the MTT method to test the proliferation rate of peripheral blood T cells and B cells. Researchers took microscope count method to calculate the total number of leukocytes in blood; injected CRCs to chicken enterocoelia to test the macrophages’phagocytosis; took xanthine oxidase method to test the SOD vitality in blood serum; took the method that count the red blood cell number of 200 phagocyte has phagocytosed and calculate devouring percentage and index of macrophage cell to test the macrophages’phagocytosis; took ELISA method to test the total antibodytiter in blood serum; took thymus, spleen and bursa weight method to test immune organ index. The results showed that in all test groups, Venenum Bufonis injection could observably increase proliferation rate of peripheral blood T cells. In the high dosage group （0.25 mg/mL） and the medium one （0.1 mg/mL）, Venenum Bufonis injection could significantly increase the total number of leukocytes in the blood. In all test groups, Venenum Bufonis injection could significantly increase devouring percentage and index of macrophage cell. In the high dosage group and the medium one of all the groups, Venenum Bufonis injection could significantly increase SOD content. In all of groups, Venenum Bufonis injection had different levels effects on promotion of Marek＇s disease virus vaccine making antibodies. In all of groups, Venenum Bufonis injection had significant effects on promotion of immune organ growth. The research indicated that Venenum Bufonis injection could enhance the immunity activity from nonspecific immunity, specific immunity, immunocyte and immune molecules. The results showed Venenum Bufonis injection was the effective immunoenhancement of Marek＇s disease virus vaccine.