响应面法优化解脂耶罗维亚酵母ypy01淀粉酶发酵条件研究

Optimization of Amylase Production from Yarrowia lipolytica ypy01 by Using Response Surface Methodology

利用Plackett-Burman(PB)设计和响应面法,对解脂耶罗维亚酵母ypy01淀粉酶生产条件进行了优化。PB设计筛选到3个显著因子,分别为可溶性淀粉浓度、蛋白胨浓度和初始pH。采用响应面法对3个显著因子进一步优化,获得了最佳培养基组成:0.84%可溶性淀粉,2.25%蛋白胨,1%酵母粉,3%NaCl,0.05%MgSO4,0.05%CaCl2,初始pH为6.8,在最优条件下,淀粉酶产量达到2012.50 U/mL。

The aim of this work was to optimize the operational parameters of marine yeastYarrowia lipolytica ypy01 to increase amylase production using Plackett-Burman （PB） design and response surface methodology （RSM）. The results of PB design showed that the concentrations of starch and peptone and initial pH were the most significant factors to influence amylase production. A Central Composite Design （CCD）, which is the standard design of RSM, was then employed to further optimize these three factors. The experimental results indicated that the optimized composition of medium was 0.84% starch, 2.25% peptone, 1% yeast extract, 3% NaCl, 0.05% MgSO4, 0.05% CaCl2 and pH 6.8. Under the optimized conditions, the amylase production was up to 2012.50 U/mL in shake flask experiments.