高矿化度条件下石油烃降解菌种的筛选与评价

High salinity conditions for screening and evaluation of the degradation of petroleum hydrocarbons bacteria

为了获得高效石油降解菌种,以原油为唯一碳源,从油水混合物中分离筛选出菌株。研究不同的温度、转速等对菌体生长情况和石油降解率的影响。在实验条件下,2株优势菌在适宜的条件下对石油的降黏率可分别高达28.5%、51.5%。偏酸或偏碱环境均不利于菌体生长,培养温度对2株菌体生长和石油降解率影响较大,最佳温度是35℃。在高矿化度条件下,菌株对原油仍有降解作用,降黏率为40%以上。原油组分分析结果表明,菌种在以原油为碳源培养后,使原油组分中沥青质、非烃及芳烃类含量均发生变化。

in order to obtain efficient petroleum-degrading bacteria, crude oil as sole carbon source,the strain was isolated from oil-water mixture. Different temperature and speed impact on cell growth and oil degradation rate. Under the experimental conditions, two dominant bacteria under appropriate conditions in the oil viscosity reduction rate can be as high as 28.5%, 51.5%. Acidic or slightly alkaline environments are not conducive to cell growth, the impact of the two oil degradation rate of cell growth is greater,the best temperature is 35 ℃. In high salinity conditions, the degradation is still on crude oil from the strain, more than 40% viscosity reduction. The result of crude oil components show that bacteria in cultured crude oil as carbon source, asphaltene fractions of crude oil, non-hydrocarbon and aromatic hydrocarbon have changed.