印度芥菜重金属ATP酶基因的分离与表达

Isolation and expression of heavy metal ATPase in Brassica juncea L.

利用同源序列克隆技术在印度芥菜幼苗中分离出2个重金属ATP酶cDNA片段BjHMA3和BjHMA4;实时荧光定量PCR表明BjHMA3和BjHMA4在所有的组织器官中均有表达,其中在根中表达量最高,叶片中表达量最少;重金属Zn或Cd胁迫均能增强二者在叶片中的表达,表明BjHMA不仅参与植物的生长发育过程,而且在重金属稳态和耐性中具有重要作用.

Brassica juncea L.is a Zn/Cd tolerance plant,and the gene expression of heavy metal ATPase（HMA） has not been reported.In the present work,two cDNA fragments of BjHMA,BjHMA3 and BjHMA4,were isolated from B.juncea seedling.Real-time quantitative PCR analysis revealed that both BjHMA3 and BjHMA4 were constitutively expressed in all tissue and preferentially in root.The mRNA profile of BjHMA3 or BjHMA4 was the lowest in leaf while the expressions were strongly enhanced by Zn or Cd stress,indicating that BjHMA played important roles in growth and in heavy metal homeostasis and tolerance in plant.