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肝纤维化大鼠SGK1的表达与肝星状细胞活化的关系及坎地沙坦对SGK1的影响 被引量：1

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摘要 目的:观察血清和糖皮质激素调节蛋白激酶1(SGK 1)的表达与肝星状细胞(HSC)活化的关系,并探讨选择性血管紧张素Ⅱ受体亚型AT l拮抗剂坎地沙坦对SGK 1的影响。方法:将36只W istar大鼠随机分为3组:正常对照组、肝纤维化模型组和坎地沙坦治疗组。采用复合法复制肝纤维化模型,坎地沙坦治疗组于第7周初时用大鼠灌胃针灌注药物,实验于10周末全部结束。苏木精-伊红染色法(HE法)和马松三色染色法(M asson法)观察肝组织病理学改变,应用免疫组织化学法检测α-平滑肌肌动蛋白(-αSM A)和SGK 1在肝内的表达及定位,并应用图像分析系统对其阳性表达结果进行半定量分析。结果:各模型组-αSM A,SGK 1的表达量较正常对照组明显增多(P<0.01);坎地沙坦治疗组-αSM A和SGK 1的表达量较同期纤维化模型组明显减少(P<0.0 5)。统计结果表明二者呈正相关(r=0.899,P<0.001)。结论:SGK 1参与了肝星状细胞的激活,坎地沙坦可以抑制肝纤维化的发展,其机制可能与HSC活化受抑、SGK 1表达减少有关。
作者 张舒静 师水生 田慧 张佑蕊
机构地区 山西医科大学第二临床医学院
出处 《临床医药实践》 2011年第7期518-520,共3页 Proceeding of Clinical Medicine
关键词 肝纤维化 糖皮质激素调节蛋白激酶1 坎地沙坦
分类号 R653.7 [医药卫生—外科学]
  • 相关文献

参考文献9

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二级参考文献22

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共引文献8

同被引文献13

  • 1姜华军,刘建社,朱忠华,王全胜,王玉梅,冯玉锡.SGK1在糖尿病小鼠肾脏皮质中时间差异性表达及意义[J].中国病理生理杂志,2007,23(11):2278-2280. 被引量：1
  • 2Waldegger S,Erdel M,Nagl UO, ez al . Genomic organization and chromosomal localization of the human SGK. protein ki- nase gene[J]. Genornics, 1998,5 (1) : 299-302.
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  • 9王全胜,刘建国,易继飞.血清和糖皮质激素诱导蛋白激酶1在梗阻性肾病肾间质中的表达和血竭素高氯酸盐对其的干预作用[J].中西医结合研究,2009,1(4):169-172. 被引量：2
  • 10李彩蓉,蔡飞,黎荣,赵辛元,李娟.氟伐他汀对醛固酮诱导的系膜细胞SGK1及CTGF表达的影响[J].中国病理生理杂志,2010,26(5):956-960. 被引量：9

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临床医药实践
2011年 第7期

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