摘要
本试验研究了大豆异黄酮对鸭小肠黏膜内淋巴细胞分布的影响。试验选用咔叽-康贝尔鸭200只,随机分成对照组和试验组。每组100只,对照组饲喂基础日粮,试验组在基础日粮中添加0.04%大豆异黄酮,试验期为40d。试验结束时从各组选择50只鸭进行屠杀,采集鸭的十二指肠、空肠、回肠组织样本,置于波氏固定液中固定。采用常规石蜡切片技术制作组织切片,应用OlympusCH-30显微摄影系统进行观察与拍照。结果表明,饲喂大豆异黄酮的试验组鸭小肠黏膜内淋巴细胞分布较对照组明显增多。说明大豆异黄酮能显著提高咔叽-康贝尔鸭小肠黏膜免疫功能。
To study the effect of soybean isoflavones on the distribution of lymphocyte in small intestine mucosal of ducks.200 KaJi-Cambel ducks were randomized into the control group and the experiment group.Each group has 100.The control was fed basal diet.Soybean isoflavones was supplemented in basal diets of experiment group by 0.04%.The experiment period was 40 days.The experiment ducks were slaughtered at the end of experiments.The samples of small intestine of every group ducks were collected.The samples were fixed in the Bouin liquid,produced the paraffin slice and H·E dyeing.The slice was observed and photograph under the microscope of OlympuCH-30.The result reveals that the intramucosal lymphocyte in small intestine of experiment group ducks were increased than the control group.Immune function of small intestine mucosal was promoted by the soybean isoflavones.
出处
《安徽科技学院学报》
2011年第2期1-4,共4页
Journal of Anhui Science and Technology University
基金
安徽省农业科技成果转化资金项目(1104b0303013)
安徽科技学院重点学科基金项目(AKXK20101-2)
关键词
大豆异黄酮
咔叽-康贝尔鸭
小肠
黏膜免疫
淋巴细胞
Soybean isoflavones
KaJi-Cambel Duck
Small intestine
Mucosal immunity
Lymphocyte