亚硝态氮和氨态氮急性胁迫下曼氏无针乌贼幼体血液的生化指标

Biochemical Parameters in Juvenile Sepiella maindroni Induced by Acute Exposure to Nitrite-N or Ammonia-N

将曼氏无针乌贼幼体置于24h半致死剂量的亚硝态氮和氨态氮溶液中(10mg·L-1NaNO2和300mg·L-1NH4Cl),检测6、12和24h各点及对照组曼氏无针乌贼幼体血液生化指标的变化。结果表明,随着处理时间的延长,NaNO2处理组血细胞密度(THC)(p<0.05)、甘油三酯(TG)(p<0.05)和谷丙转氨酶(GPT)(p<0.05)均呈"先降后升"的变化趋势,这与谷草转氨酶(GOT)(p<0.05)和GOT/GPT比率(p<0.05)的变化趋势相反;血糖(GLU)呈"升降升"(p>0.05)的趋势;总胆固醇(CH)呈"逐步下降"(p>0.05)的趋势,这与总蛋白(T-MP)(p<0.05)的变化趋势相反。NH4Cl处理组THC(p<0.05)、GPT(p<0.05)和GOT(p<0.05)均呈"逐步降低"的变化趋势,这与GOT/GPT比率(p<0.05)的变化趋势相反;GLU呈"降升降"(p<0.05)的变化趋势,这与CH(p<0.05)、TG(p<0.05)和T-MP(p<0.05)的趋势相反。据此推测,亚硝态氮和氨态氮胁迫下,血液生化指标变化趋势的差异可能与这两种毒物自身的作用特点有关。肝脏受到亚硝态氮的毒害要大于氨态氮,但这也并非机体死亡的主要原因。蛋白与血糖在消除氨态氮时发挥着互相补充的作用,且与总胆固醇和甘油三酯一样可以作为氨态氮胁迫的指示物。

To better understand the effects of nitrite-N and ammonia-N on blood of the juvenile Sepiella maindroni,Juvenile Sepiella maindroni were exposed to 10 mg·L-1 sodium nitrite or 300 mg·L-1 ammonium chloride for 0,6,12 and 24 hours respectively.The blood immune parameters-total haemocyte count（THC）,glucose（GLU）,cholesterol（CH）,triglyceride（TG）,total protein（T-MP） concentrations,glutamic pyruvic transaminase（GPT） and glutamic oxaloacetic transaminase（GOT） activities,GOT/GPT ratios were investigated in this study.The results show that THC（p0.05）,TG concentration（p0.05） and GPT activity（p0.05） first fell then rose,running counter to that of GOT activity（p0.05） and GOT/GPT ratio（p0.05）;GLU concentration（p0.05） first rose then fell and rose again at 24 h;CH concentration（p0.05） decreased significantly,running counter to that of T-MP（p0.05） with the elapse of nitrite-N exposure time.With the elapse of ammonia-N exposure time,THC（p0.05）,GPT（p0.05） and GOT activities（p0.05） decreased significantly,running counter to that of GOT/GPT ratio（p0.05）;GLU concentration（p0.05） first fell then rose and fell again at 24 h,running counter to that of CH（p0.05）,TG（p0.05） and T-MP（p0.05）.The results suggest that the differences of blood biochemical parameters between nitrite-N and ammonia-N result from their characteristics.Liver was more seriously damaged caused by nitrite-N than ammonia-N,however,that was not the main reason for death.Protein and GLU were complementary in eliminating ammonia-N,and which can be used as biomarkers of exposure to ammonia-N with CH and TG.