家蚕胚胎发育时期的蛋白质变化及构造分析

Characterization and Stage-specific Change of Proteins During the Embryonic Development of Silkworm Bombyx mori

采用蛋白质双向电泳技术及蛋白质氨基酸序列分析技术，从蛋白质水平研究了家蚕胚胎发育时期的基因表达情况。结果表明，从家蚕临界期胚胎直到点青期胚胎的较长一段时间内，蛋白质的双向电泳图谱变化不大，匹配蛋白质斑点比率达63．0％，卵特异性蛋白质，30K蛋白质的含量很大。由点青期胚胎到转青期胚胎，再到蚁蚕期，胚胎发育的时间虽然很短，但蛋白质双向电泳图谱的变化却很大，匹配蛋白质斑点比率下降，偏酸性蛋白质明显增多，卵特异性蛋白质、30K蛋白质的含量逐渐消失，类似于幼虫血液蛋白、烯醇化酶蛋白、以及超氧化物歧化酶蛋白的基因被激活和表达。

The gene expression pattern was analysed employing protein two-dimensional electrophoresis and amino acid sequencing during the embryonic development of silkworm Bombyx mory. The 2D-PAGE pattern of the silkworm embryonic proteins had little change in the long developmental Period from the Critical Stage until the Head Pigmentation Stage of silkworm embryo. The Percentage of matched proteins that found betWeen the spots in the 2D-PAGE pattern of the Critical Stage of silkworm embryo and the spots in the 2D-PAGE pattern of the Head Pigmentation Stage of silkworm embryo was 63%. Silkworm Egg-specific Protein and 30K Protein were in large amount However, 2D-PAGE pattern of the silkworm embryonic proteins showed considerably varied in shorter developmental stage from the Head Pigmentation Stage to the Body Rgmentation Stage and until the Newly Hatched Larva. The percentage of matched proteins that found the spots in the 2D-PAGE pattern of each developmental stage of silkworm embryo became lesser. The protein spots that the isoelectric points lying on th acidic side greatly increased, the Silkworm Egg-specific Protein and 30K protein were gradually disappeared and the gene that resembles BmLSP's, Enolase's and Superoxide dismutase's gene was activated and expressed with the embryonic development.