摘要
目的建立高效液相色谱法，用于测定人血浆中连翘苷的质量浓度。方法采用内标法，以Thermo Cl8柱（4．6mm×250mm，5μm）为固定相，以乙腈-水-冰醋酸（17：83：0．4）为流动相，流速为1mL／min，检测波长为277nm。结果血浆中连翘苷质量浓度在0．10～4．00μg／ml范同内与峰面积比值线性关系良好（R2=0．999），最低检测浓度为0．05μg／ml，回收率达90％以上。结论该方法具有较高的准确度，线性范围宽，方法灵敏，专一性好，操作简便，适用于连翘苷血药浓度的测定及临床药代动力学研究。
Objective To determine the concentration of Phillyrin in human plasma by HPLC with ultraviolet detetion. Methods The internal standard method was used. The evaluation of Phillyrin was car ried out by ThermoC 18 colum （4.6mm × 250mm, 5 μm）with the mobile phase ofAcetonitrile - water - acetic acid （17 ： 83：0.4 ） and the detection wavelength was 277nm. The flowrate was 1 mL / min. Results The calibration curve for plasma Phillyrin in range of 0.10 - 4.00 μg / ml （R2= 0.999）. The detection limit of Phillyrin was 0.05 μg/ml. The recovery was more than 90%. Conclusion This method is accurate,sensitive,specific and convenient,which could be used in the determination of plasma Phillyrin and its pharmacokinetic study in clinic.
