壳聚糖衍生物的制备及其抑菌活性的研究

Preparation and antibacterial activity determination of chitosan derivatives

改进壳聚糖衍生物制备技术,并用红外光谱对其结构进行表征;并测定了所合成衍生物的稳定性、溶解性及pH值等相关性质,同时用改进后的CS衍生物进行抑菌实验的初步研究。结果表明经过技术改进制备的3种不同分子量的CS衍生物具有良好水溶性且稳定性增强。两种CS衍生物在0.1%(w/v)处对大肠杆菌和金黄色葡萄球菌具有抑制作用,到0.2%(w/v)基本可以完全抑制菌体生长。分子量大的CS衍生物对革兰氏阳性菌的抑菌作用强,而分子量小的CS衍生物对于革兰氏阴性菌抑菌作用强。

The chitosan derivatives were improved in preparation method and characterized by FT IR spectrum a nalysis. The bacteriostasis, stability, dissolubility, pH and other properties of the chitosan derivatives were in vestigated. The experimental results indicated that the dissolubility and stability of the chitosan derivatives pre pared by the improved method were prominently enhanced. Two kinds of the chitosan derivatives at the concen tration of 0. 1~ （w/u） showed obvious bacteriostatic activities on Esherichia coli and Staphylococcus aureus. Their growths were terminated when the concentration reached 0. 2%（w/u）. The bacteriostatic activity of high molecular weight （Mw） chitosan derivative is higher on gram positive microbes while that of low Mw chitosan de- rivative is higher on gram negative bacteria.