摘要
目的 研究ＣＤＫＮ２ 基因的突变、缺失与原发性胃癌发生发展的关系。方法 用ＰＣＲ和银染ＳＳＣＰ分析了４５ 例胃癌组织和２２ 例相应正常胃粘膜中ＣＤＫＮ２ 基因的突变和纯合性缺失情况。结果 发现ＣＤＫＮ２ 基因的纯合性缺失率为２８－９ ％ ，其中β型转录子的缺失率为１５－６ ％ ；无一例有ＣＤＫＮ２ 基因的突变。结论 胃癌的发生发展与ＣＤＫＮ２ 基因纯合性缺失密切相关，与ＣＤＫＮ２基因的突变无关，β型转录子参与维持ＣＤＫＮ２ 基因的正常功能，其纯合性缺失与胃癌的发生有关。
Objective In order to study the alterations of CDKN2 gene in human gastric cancer.Methods The deletion and mutation of CDKN2 gene in 45 gastric carcinoma and 22 normal gastric tissues were examined using PCR SSCP analysis.Results 28 9%(13/45 cases)deletion were founded in gastric cancer,but no somatic mutation was present.Conclusion The findings suggested that CDKN2 gene deletion play a important role in the gastric tumorigenesis,whereas CDKN2 mutation is absent.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1999年第5期348-349,352,共3页
Cancer Research on Prevention and Treatment
关键词
CDKN2基因
胃癌
突变
抑癌基因
CDKN2 gene
Gastric carcinoma
Homozygous deletion
Mutation
