大黄提取液抗柯萨奇病毒B_3的实验研究

Anti-Coxsackievirus B_3 Activity of Rheum Offinicale Extraction in Vitro

采用微量细胞培养法观察细胞病变效应 (Cytopathiceffect,CPE) ,测定经不同剂量大黄提取液作用后的病毒滴度 ,用逆转录—聚合酶链反应 (RT PCR)检测药物作用后细胞冻融液中的病毒核酸(CVB RNA) ,研究大黄提取液的抗柯萨奇病毒B组 (CVB)作用 ,探讨其抗病毒作用机制。结果 :不同剂量大黄提取液和病毒作用后 ,细胞均出现CPE ;不同剂量药物作用于细胞后 ,不能阻止CVB3感染 ;当CVB3感染细胞后 ,大黄提取液能阻止病毒增殖 ,并表现出一定的量效关系 ,其治疗指数(TI) =10。提示大黄提取液不能直接杀灭CVB3,不能阻止CVB3吸附、穿入易感细胞 ,而通过阻止CVB3核酸复制或 /和以后环节发挥抗病毒作用。

To research antiviral effect of Rheum offinicale extraction on coxsackievirus B 3 (CVB 3 ) and elucidate its antiviral mechanism. Cytopathic effect (CPE) was observed and infectious virus was titered by micro well cells culture, CVB RNA was detected by reverse transcription polymerase chain reaction (RT PCR) in Hep 2 cells supernatant. The results showed CPE can not be observed, infectious viral titer is less than 1 logarithm unit and CVB RNA is detectable in 10 -3 dilution of viral titered microwell at 8 μg/mL. These results suggested that Rheum offinicale extraction can interrupt viral biosynthese other than killing directly CVB 3 or interfere in viral absorption and penetrance, especially can interrupt viral nucleic acid amplification or/and the last stage of viral replication cirle.