贵州地方猪品种脂蛋白脂酶基因第8内含子的多态性研究

Polymorphism of the Eighth Intron in Lipoprotein Lipase Gene of Guizhou Indigenous Pig Breeds

以可乐猪、萝卜猪和香猪3个贵州地方猪种和欧洲猪种为材料,采用PCR-SSCP技术对脂蛋白脂酶(Lip-oprotein lipase,LPL)基因第8内含子的多态性进行了研究。以特异性PCR从样品基因组中扩增出163bp的DNA片段,经非变性聚丙烯酰胺凝胶电泳,从两个猪群体的LPL基因第8内含子中都检测到了AA基因型和AB基因型,未检测到BB基因型;欧洲猪品种和贵州地方猪品种均以AB基因型占优势;与欧洲猪品种相比,贵州地方猪品种的B等位基因频率明显较高,A等位基因频率则较低。

Taking the Kele,Luobo and Xiang pig breeds and the European pig breeds as samples,the polymorphism of lipoprotein lipase（LPL） gene was investigated using single-strand conformation polymorphism（SSCP） method.A DNA fragment of 163 base pairs（bp） was amplified from the genomic DNA of blood sample of pig breeds and classified by native polyacrylamide gel electrophoresis.Both of AA and AB except BB genotypes were detected and the AB genotype was dominant in all pig breeds.Compared with European pig breeds,Guizhou indigenous pig breeds contained a higher B allele percentage and a lower A allele.In previous reports,a Hind III site in the intron 8 of LPL gene was ascertained to be the binding site of transcript factor and its mutation was confirmed to be related with the triglyceride level in blood of human and meat quality in Rongcang pig.The Hind III site was included in the 163 bp-fragment amplified in the present study based on alignment of nucleotide sequence of LPL.It suggested that the dominant B allele might be pivotal for the better meat quality of Guizhou pig breeds.High percentage of allele B would improve the meat quality of pig individuals with taking advantage of the precious resources of Guizhou pig breeds.