分子印迹聚合物固相萃取检测食品中痕量雌二醇

Detection of Trace 17β-estradiol in Food Samples Using Molecularly Imprinted Solid-phase Extraction

目的利用雌二醇分子印迹聚合物固相萃取（MISPE）结合液相色谱紫外检测（HPLC-UV）方法,检测奶类和肉类食品中痕量雌二醇。方法采用改良沉淀聚合法,制备雌二醇分子印迹聚合物（MIPs）,对各种奶类和肉类样品进行MISPE,结合HPLC-UV,测定各种样品中痕量雌二醇。结果改良沉淀聚合法可以得到规律的MIPs微球,对雌二醇有特异性识别和结合能力。在最佳固相萃取条件下,MISPE结合HPLC-UV法,可准确测定500 g奶类和肉类食品中痕量雌二醇（0.116~0.461 nmol/kg）。结论 MISPE结合HPLC-UV方法可用于食品中痕量雌二醇的常规性检测。

Objective Molecularly imprinted solid-phase extraction was used to detect trace 17β-estradiol（E2）in food samples.Methods E2-imprinted molecularly imprinted polymers prepared by modified precipitation method were used as SPE sorbents for selectively trapping and pre-concentration of 17β-estradiol from different food samples.High performance liquid chromatography（HPLC）was used to detect the extracted E2.Results Trace（0.116-0.461 nmol/kg）E2 in different dairy and meat samples could be detected accurately when large amount of sample（500 g）was analyzed.Conclusion Molecularly imprinted solid-phase extraction is a reliable method to detect trace E2 in food samples with high accuracy and repeatability.