冬虫夏草菌丝体水提物的抗新城疫病毒作用

Effects of water extract from Cordyceps sinensis mycelium on anti-newcastle disease virus

通过低温水浸提取方法,获取冬虫夏草菌丝体水提物。采用MTT的方法和观察细胞病变(CPE)的方法分别研究了水提物毒性和抗病毒作用。结果显示水提物浓度≤0.5mg/mL时,对Vero细胞无毒性,0.5mg/mL水提物与新城疫病毒作用,或预处理Vero细胞,均能抑制新城疫病毒在细胞上病变的形成。提示水提物具有直接抗新城疫病毒和预防新城疫病毒感染的作用。

The water extract was obtained by aqueous leaching in low-temperature from Cordyceps sinensis mycelium.The toxicity and antiviral activity of water extract were investigated by using MTT method and observing cytopathic（CPE）,respectively.The results showed that the concentration of water extract≤ 0.5mg/ml was no toxicity on Vero cells.Either pre-mixed the water extract with newcastle disease virus or with Vero cells for 1 hour before cell cultivation assay was able to inhibit cytopathic effect of newcastle disease virus significantly.The research indicates that the water extract has direct and preventive effect against newcastle disease virus infection.