宫颈癌及癌前病变中IL-10表达和HPV感染的相关性研究

Expression of IL-10 in cervical cancer and precancerous lesion and its correlation with HPV infection

目的:研究宫颈疾病病灶中IL-10表达与高危型人类乳头瘤病毒(HPV)感染情况及其相关性,从而了解宫颈疾病病灶局部免疫逃逸的可能机制。方法:收集118例宫颈分泌物及宫颈组织,其中浸润性宫颈鳞癌40例,22例宫颈上皮内瘤变(CIN)1,18例CIN2,18例CIN3,正常女性宫颈组织20例。采用第二代杂交捕获法检测与宫颈感染高危型HPV的情况,RT-PCR法测定宫颈组织IL-10 mRNA的表达。结果:在正常宫颈、CIN1、CIN2-3和宫颈浸润癌组织中,高危HPV感染的比例依次为5.00%、40.91%、83.33%和100.00%。IL-10 mRNA表达率依次为0、18.18%、52.78%和87.50%,在高危HPV感染的CIN1、CIN2-3和宫颈浸润癌组织中的比例依次为44.44%、63.33%和87.50%。各组之间差异有统计学意义,P<0.05。结论:IL-10抑制宫颈局部免疫反应是宫颈HPV感染导致宫颈癌前病变及宫颈癌发生发展的重要因素,IL-10的表达与HPV和子宫颈癌疾病的阶段有明显相关性,可能有助于肿瘤部位微环境的免疫抑制作用。

OBJECTIVE： To determine IL-10 expression in cervical tissues in women with cervical cancer and CIN and its association with high risk HPV infection. METHODS： Totally 118 cases of cervical tissues were involved in this study, including 40 squamous carcinoma of the cervix, 22 CIN1, 18 CIN2, 18 CIN3, and 20 normal tissues. High risk HPV infection was detected by hybrid capture 11, and IL-10 expression was determined by RT-PCR. RESULTS： High risk HPV and IL-10 showed a clear tendency to increase during the different cervical disease stages： 5.00% and 0 in normal cervix, 40. 91% and 18.18% in CIN1, 83.33% and 52.78% in CIN2-3, 100.00% and 87.50% in cancer. All the patients that expressed IL-10 were HPV positives. The proportions of IL-10 in HPV infected CIN1, CIN2-3 and CC were 44.44%, 63.33%, 87. 50% in turns. The differences between different groups were significant （P〈0.05）. CONCLUSION： IL-10 expression and HPV and cervical disease stage, may help to have obvious concer tumor site microenvironment immune inhibition.