脂多糖刺激小鼠成骨细胞表达白细胞介素-23的实验研究

The in vitro study of LPS-induced IL-23 expression in murine osteoblast

目的研究小鼠成骨细胞能否在细菌毒素产物脂多糖刺激下表达白介素-23(IL-23)。方法分别以小鼠骨髓基质干细胞(BMSCs)及小鼠成骨前体细胞系MC3T3-E1体外诱导为成骨细胞后,加以10ng/ml LPS刺激,采用ELISA法检测IL-23的蛋白表达。并采用RT-PCR方法检测脂多糖刺激下小鼠BMSCs诱导成骨细胞IL-23两亚基p19和p40 mRNA的表达。结果 10ng/ml脂多糖刺激下,小鼠BMSCs诱导成骨细胞和MC3T3-E1诱导成骨细胞均表达IL-23蛋白,BMSCs诱导成骨细胞表达IL-23两亚基p19及p40 mRNA。结论脂多糖能刺激小鼠成骨细胞表达IL-23。

Objective To examine if murine osteoblasts could express IL-23 when stimulated by lipopolysaccharide（LPS）.Methods Murine bone marrow stromal cells（BMSCs） and MC3T3-E1 cells were cultured to drive osteoblasts in vitro,and stimulated by LPS.ELISA was used to test IL-23 protein expression in BMSCs and MC3T3-E1 derived osteoblasts.RT-PCR was used to examine the two chain of IL-23,p19 and p40 mRNA expression in BMSCs derived osteoblasts.Results Both BMSCs and MC3T3-E1 derived osteoblasts expressed more IL-23 protein than control（P0.05）.The two chain of IL-23,p19 and p40 mRNA were expressed in BMSCs derived osteoblasts.ConclusionOsteobalst could express IL-23 when stimulated by LPS.