大鼠大网膜I型和Ⅱ型乳斑的确定及对比

Identification and comparison between omental type I and type II milky spots of the greater omentum in rat

目的：对乳斑的分型进行确定，并探讨I型乳斑和Ⅱ型乳斑数量、面积及细胞构成的异同。方法：微粒子活性炭及H—E染色双重标记大鼠大网膜乳斑，Image-ProPlus软件测定I型乳斑和Ⅱ型乳斑的数量和面积，电镜观察I型乳斑和Ⅱ型乳斑的超微结构。结果：生理状态下，Ⅱ型乳斑数量（9．153／cm^2±0．818／cm^2）多于I型乳斑数量（1．913／cmz±0．851／cm^2），Ⅱ型乳斑面积（O．421mmz±0.136mm^2）大于I型乳斑面积（0．102mm^2±0．041mm^2）。两型乳斑均主要由巨噬细胞、淋巴细胞、肥大细胞、单核细胞、浆细胞、嗜酸性粒细胞、网状细胞和基质细胞构成；Ⅱ型乳斑内还可见到血管、淋巴管和脂肪细胞。两型乳斑巨噬细胞内均见吞噬的微粒子活性炭颗粒。结论：微粒子活性炭与H—E染色均可标记乳斑；大网膜乳斑分为两种类型即I型和Ⅱ型，I型乳斑分布于薄的大网膜浆膜，周围无毛细血管和脂肪组织，Ⅱ型乳斑分布于网膜血管和脂肪组织周围，乳斑内可见曲迂的血管；两型乳斑在组织细胞构成方面有差异，与它们的分布位置有关；两型乳斑巨噬细胞均有吞噬异物颗粒的免疫功能。

Objective： To identify the type of milky spots, and investigate the similarities and differences between type I and type Ⅱ milky spots in number, area and cell composition. Methods： Double-labeling of rat omental milky spots with activa- ted carbon and H-E staining were used to measure the number and the area of type I and type Ⅱ milky spots by Image-Pro plus software, and the differences in ultrastructure between type I and type Ⅱ milky sports were observed under an electron microscope. Results： In physiological conditions, the number of the type Ⅱ milky spots was more significant than that of the type I milky spots [（9. 153q±0. 818）per cm^2 vs （1. 913±0. 851）per cm^2 , P〈0. 011, and the area of the type Ⅱ milky spots was significantly larger than that of the type I milky spots [（0. 421±0.136）mm^2 vs （0. 102±0. 041）mm^2, P 〈0.01]. Two types of milk spots were mainly composed of macrophages, lymphocytes, mast cells, monocytes, plasma cells, eosinophils, reticular cells and stromal cells; vessel, lymph vessel and adipocyte were observed in type Ⅱ milky spots. Activated carbon particles were observed in macrophages of two types milky spots. Conclusion： Both activated carbon and H-E staining can mark milky spots; Omental milky spots can be classified into two types., type I and type Ⅱ （type I milky spots are located in the thin serous membrane of the omentum and no blood vessels or adipose tissues are found in the surrounding area; type Ⅱ milky spots are distributed close to vascular networks and adipose tissues, curved vessel can be observed in this milky spots）. Two types of the milky spots in the tissue cell composition are different due to the distribution; Macrophages in both types of milky spots have the immune function of phagocytizing foreign particles.