摘要
目的:探讨二烯丙基二硫(DADS)与硼替佐米(bortezomib)联合应用诱导人成神经细胞瘤SH—SY5Y细胞凋亡以及活性氧(ROS)的变化。方法:分别以DADS、硼替佐米单药和联合用药处理成神经瘤细胞SH—SY5Y细胞株,采用四甲基偶氮唑盐(MTT)比色法测定对细胞增殖的影响,Hoechst-33528染色观察细胞凋亡形态,流式细胞术检测细胞凋亡以及细胞内ROS的变化。结果:DADS和硼替佐米联合应用可使SH—SY5Y细胞的凋亡急剧增加,它们的联合应用还使细胞内ROS的生成比任何单一用药都多。结论:硼替佐米可促进DADS诱导SH—SY5Y细胞发生凋亡,ROS的产量增加可能在其中发挥了重要作用。
To explore the apoptosis of neuroblastoma cells and the change of ROS induced by diallyl disulfide (DADS) in combination with bortezmib. Methods: Neuroblastoma SH-SYSY cells were treated with DADS, bortezomib and DADS plus bortezomib. The cell viability was detected by methyl thiazolyl tetrazolium (MTT), and Hoechst-33528 stainning was employed to observe the apoptosis morphology. The production of ROS and the apoptotic rate of cells were measured by a flow cytometry method. Results: The combination of DADS and bortezomib increased the apoptosis of neuroblastoma cells, and their combination produced more ROS than when either was used alone. Conclusion: Bortezomib can facilitate the apoptosis of neuroblastoma induced by DADS; The enhanced production of ROS plays an important role during this process.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2011年第3期354-357,387,共5页
Chinese Journal of Anatomy
基金
山东省自然科学基金(2009ZRB01288)
滨州医学院科研计划与科研启动基金(BY2009KJlO)