系统性红斑狼疮患者血清MMP-3与TIMP-1的检测

The measurement of MMP-3 and TIMP-1 serum level in patients with systemic lupus erythematosus

目的:探讨基质金属蛋白酶-3(MMP-3)以及金属蛋白酶组织抑制因子-1(TIMP-1)在系统性红斑狼疮(SLE)中的作用和意义。方法:采取双抗体夹心ELISA法检测45名SLE患者及30名正常对照者血清MMP-3、TIMP-1水平。结果:①与正常对照组相比患者血清MMP-3水平明显增高(P<0.01);经糖皮质激素治疗后血清MMP-3浓度下降(P<0.01)。②SLE患者中血清MMP-3水平活动组高于非活动组(P<0.05),肾损害组、肺炎组、抗ds-DNA阳性组高于对照组(P<0.01),低补体C3血症者及低血清白蛋白血症者血清MMP-3水平高于非低补体C3血症者及低血清白蛋白血症者(P<0.05)。关节炎与非关节炎患者血清MMP-3水平差异无显著性(P>0.05)。③血清TIMP-1水平在SlE组与对照组、SLE相关各组间差异均无显著性(P>0.05)。④患者血清MMP-3水平与SLE疾病活动指数SLEDAI呈正相关(P<0.01),与TIMP-1无明显相关性(P>0.05)。结论:MMP-3可能参与SLE发病机制。

Objective： To explore the clinical significance of serum matrix metalloproteinase-3（MMP-3） and tissue inhibitor of metalloproteinase-1（TIMP-1） in patients with systemic lupus erythematosus（SLE）.Methods： The serum concentration of MMP-3 and TIMP-1 were measured by ELISA in systemic lupus erythematosus patients and healthy controls.Results： Firstly,the serum levels of MMP-3 in SLE patients were significantly higher as compared with that in healthy controls（P0.01）.After treatment with steroid,serum MMP-3 levels were markedly decresed.Secondly in patients with active SLE and renal involvement,pulmonary involvement,decreased C3 levels,hypoalbuminemia,anti-ds-DNA positive,serum MMP-3 levels were makedly increased than those of inactive SLE and without renal involvement,pulmonary involvement,decreased C3 level,hypoalbuminemia and anti-ds-DNA negative respectively.Serum MMP-3 levels had no significant difference in patients with arthritis and without.Thirdly,serum TIMP-1 levels did not show significant difference betwen patients with SLE and healthy controls and betwen different groups of SLE.Last,a significantly positive correlation betwen MMP-3 levels and SLEDAI scores was found in patients with SLE（P0.01）,but MMP-3 had no correlation with TIMP-1（P0.05）.Conclusion： MMP-3 may be involved in the pathogenesis of SLE,and MMP-3 may be used as indicators to moitor disease activity,renal damage,disease progression and improvement in SLE.