胃癌高表达基因gcys-18的部分cDNA克隆

Cloning part cDNA of gcys 18 overexpressed in gastric carcinoma

目的：分离克隆测序胃癌高表达基因ｇｃｙｓ１８ 的部分ｃ Ｄ Ｎ Ａ． 方法：采用ｍ Ｒ Ｎ Ａ 差异展示技术从胃癌 Ｇ Ｃ７９０１ 与胃粘膜 Ｇ Ｅ Ｓ１ 细胞株中分离出一差异表达基因片段，对其命名，并进行克隆、测序及 Ｇｅｎ Ｂａｎｋ 检索；以此片段作探针与细胞株总 Ｒ Ｎ Ａ 进行打点杂交． 结果：从差示 Ｐ Ｃ Ｒ 电泳凝胶中分离出大小为４１６ ｂｐ 的差异表达片段，命名为ｇｃｙｓ１８； Ｒ Ｎ Ａ 打点杂交证实ｇｃｙｓ１８ 在 Ｇ Ｃ７９０１ 中呈高表达，在 Ｇ Ｅ Ｓ１ 中呈低表达，表明ｇｃｙｓ１８ 是真正差异表达片段；对ｇｃｙｓ１８ 进行了序列分析及 Ｇｅｎ Ｂａｎｋ 检索，示其为新序列后递交给 Ｇｅｎ Ｂａｎｋ，它的 Ｇｅｎ Ｂａｎｋ 接受号为 Ａ Ｆ０７１０５７． 结论：分离并克隆了胃癌高表达基因ｇｃｙｓ１８ 的部分ｃ Ｄ Ｎ Ａ，此基因可能与胃癌发生发展有关．

AIM: To isolate, clone and sequence part cDNA of gcys 18 overexpressed in gastric carcinoma. METHODS: gcys 18, isolated from differential display gel between GC7901 and GES 1 by mRNA DD PCR, was cloned into T vector. As a probe, gcys 18 was hybridized to total RNAs of GC7901 and GES 1. It was then sequenced and searched against GeneBank. RESULTS: gcys 18 was isolated, cloned and found to be overexpressed in GC7901 and downexpressed in GES 1 by RNA dot blot. By sequencing, gcys 18 was confirmed to be 416 bp, partly similar to that of HEK5, and its accepted number in GeneBank is AF071057. CONCLUSION: Part cDNA of gcys 18 is isolated and cloned and it may be an important overexpressed gene in gastric carcinoma.