肺癌组织p53和K-ras基因突变及其表达产物检测的意义

Detection of the mutations of p 53 and K ras genes and their expression products

目的：研究肺癌组织中ｐ５３ 和 Ｋｒａｓ 基因突变、蛋白表达，以及二者的关系． 方法：应用 Ｐ Ｃ Ｒ Ｓ Ｓ Ｃ Ｐ和免疫组织化学方法分别检测肺癌组织中的ｐ５３ 和 Ｋｒａｓ基因突变和蛋白表达． 结果：肺癌组织中检测到ｐ５３ 和 Ｋｒａｓ基因的突变分别为５３％ 和１４％ ，肺癌组织中检测到ｐ５３ 蛋白和 ｐｒａｓ ２１ 蛋白的积聚分别为５８％ 和１９％ ． 结论：ｐ５３ 和 Ｋｒａｓ基因突变与肺癌发生关系密切，其基因突变与蛋白的积聚之间存在一致的关系，但并非完全对应． ｐ５３ 和 Ｋｒａｓ基因突变及其表达产物的检测对肺癌患者有一定的辅助诊断意义．

AIM: To investigate the mutations and the products of p53 and K ras genes in lung cancer tissues and to study the relationship between gene mutation and oncoprotein expression. METHODS: PCR SSCP and immunohistochemistry were used to detect p 53 and K ras gene mutations and oncoprotein expressions. RESULTS: 53% and 14% of the lung cancer tissues were detected to have p 53 and K ras gene mutations and 58% and 19% have p53 and p ras 21 proteins accumulation. CONCLUSION: The genesis of lung cancer is related with p 53 and K ras genes mutations. Though the gene mutation is closely related to the protein accumulation. The detection of p 53 and K ras genes and their expression products is helpful in the diagnosis of lung cancer.