抗肿瘤抗生素云南霉素诱导人肝癌HepG_2细胞凋亡

Yunnanmycin Induces Apoptosis in Human Liver Carcinoma HepG_2 Cells.

目的观察抗肿瘤抗生素云南霉素对人肝癌HepG2细胞凋亡的影响。方法体外培养人肝癌细胞株HepG2,MTT法检测云南霉素对细胞增殖的影响,Hoechest33342法检测细胞凋亡的形态变化,Western blotting法检测凋亡相关蛋白PARP的表达,AnnexinⅤ-FITC/PI双染结合流式细胞仪检测细胞的凋亡率。结果云南霉素对HepG2细胞增殖抑制的IC50值为24.13μmol/L;HepG2细胞经云南霉素作用24h后,可诱导胞内凋亡标志蛋白PARP的切割,并随浓度的增高而作用增强;而HepG2细胞经云南霉素作用48h后,可出现典型的凋亡形态变化,呈剂量依赖性引起细胞凋亡。结论云南霉素可抑制人肝癌细胞株HepG2的增殖,诱导细胞发生凋亡。

Objective To observe the apoptosis inducing effect of yunnanmycin in human liver carcinoma HepG2 cells. Methods HepG2cells were cultured in DMEM media. After corresponding treatment of yunnanmycin, the cell proliferation was assayed by MTT. The apoptotic rate was measured by flow eytometry analysis. The apoptotic nucleus was evaluated by Hoechst33342 staining, and the expressions of PARP were determined by Western blotting. Results The ICs0 value for HepG2cells was 24.13μmol/L. With the increasing concentration of yunanmycin after 24h, the expressions of apoptotic protein PARP cleavage were significantly increased. When cells were treated at different concentrations for 48h, typical morphlogy of apoptotic nuclei was observed in many cells, and the apoptotic rate was signifi- cantly raised. Conclusion Yunnanmycin could inhibit the proliferation of HepG2cells and induce apoptosis.